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Genetic suppression of the RNA regulator system controlling virulence and antibiotic biosynthesis in the phytopathogen Erwinia carotovora

控制植物病原体胡萝卜软腐欧文氏菌毒力和抗生素生物合成的 RNA 调节系统的基因抑制

基本信息

批准号：
BB/F009666/1
负责人：
George Salmond
金额：
$ 40.34万
依托单位：
University of Cambridge
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2008
资助国家：
英国
起止时间：
2008 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FF009666%2F1
关键词：
Genetic suppression RNA regulator system

项目摘要

This project involves a study of the control of multiple virulence factors in the bacterial plant pathogen, Erwinia carotovora. This bacterium causes soft rot and blackleg diseases of potato and it is commercially important. The bacteria make and secrete a collection of enzymes that degrade plant cell walls. This is the main, but not sole, mechanism of plant attack. The bacteria can 'talk' to each by chemical signalling in a process called 'quorum sensing'. By this mechanism the bacteria keep production of the plant degrading enzymes at a low level until the bacterial population reaches a high density when the bacteria secrete a lot of the enzyme in a burst of expression. This gives the plant no time to resist the lethal effects of the infection and leads to a very productive attack of the plant by the bacterial pathogen. A key control mechanism for the plant degrading enzymes is a system called the RsmAB system. The two factors that act in this RsmAB system are RsmA (a small protein) and rsmB, which is a small RNA molecule. The RsmA protein acts by degrading the products of the plant cell wall degrading enzyme genes. However, the rsmB molecules sequesters the RsmA protein to prevent it from assisting the degradation of the messenger molecules that make the enzymes. Bacterial mutants that cannot make the rsmB molecule have an excess of RsmA and this leads to excessive degradation of the messages that make the plant cell wall degrading enzymes. The rsmB mutants are not virulent because they make very little enzymes. We have exploited this fact to identify a totally new gene that, when mutated, can bypass (suppress) the effects of the rsmB mutation. This new suppressor gene is called rsmS and it nullifies the effect of the rsmB mutation. Our aims are to study the nature and the effects of the new rsmS gene, We intend to investigate the impacts of rsmS by the use of genetics, DNA microarray technology (transcriptomics) and advanced proteomics. We hope to understand how the new rsmS suppressor works to impact on the control of plant cell wall degrading enzymes in Erwinia. This information could lead eventually to a deeper understanding of regulation of pathogenicity in the plant pathogen. Ultimately, this type of information might be exploited as a route to intervening rationally in the plant disease.

该项目涉及控制细菌性植物病原体胡萝卜软腐欧文氏菌（Erwinia carotovora）中多种毒力因子的研究。该细菌引起马铃薯软腐病和黑胫病，具有重要的商业价值。这种细菌产生并分泌一系列降解植物细胞壁的酶。这是植物攻击的主要机制，但不是唯一机制。细菌可以通过一种称为“群体感应”的化学信号与每个人“交谈”。通过这种机制，细菌将植物降解酶的产量保持在低水平，直到细菌群体达到高密度，此时细菌以爆发式表达分泌大量酶。这使植物没有时间抵抗感染的致命影响，并导致细菌病原体对植物的非常有效的攻击。植物降解酶的关键控制机制是称为RsmAB系统的系统。在这个RsmAB系统中起作用的两个因子是RsmA（一种小蛋白质）和rsmB（一种小RNA分子）。RsmA蛋白通过降解植物细胞壁降解酶基因的产物起作用。然而，rsmB分子螯合RsmA蛋白，以防止其协助降解产生酶的信使分子。不能产生rsmB分子的细菌突变体具有过量的RsmA，这导致产生植物细胞壁降解酶的信息的过度降解。rsmB突变体没有毒性，因为它们产生的酶很少。我们已经利用这一事实来鉴定一种全新的基因，当突变时，它可以绕过（抑制）rsmB突变的影响。这种新的抑制基因被称为rsmS，它消除了rsmB突变的影响。我们的目的是研究新的rsmS基因的性质和作用，我们打算利用遗传学，DNA微阵列技术（转录组学）和先进的蛋白质组学来研究rsmS的影响。我们希望了解新的rsmS抑制剂如何影响欧文氏菌中植物细胞壁降解酶的控制。这些信息最终可能导致更深入地了解植物病原体的致病性调节。最终，这种类型的信息可能被利用作为合理干预植物疾病的途径。

项目成果

期刊论文数量（9）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

A Plasmid-Transposon Hybrid Mutagenesis System Effective in a Broad Range of Enterobacteria.

DOI：
10.3389/fmicb.2015.01442
发表时间：
2015
期刊：
Frontiers in microbiology
影响因子：
5.2
作者：
Monson R;Smith DS;Matilla MA;Roberts K;Richardson E;Drew A;Williamson N;Ramsay J;Welch M;Salmond GP
通讯作者：
Salmond GP

Identification of genes in the VirR regulon of Pectobacterium atrosepticum and characterization of their roles in quorum sensing-dependent virulence.

黑腐果杆菌 VirR 调节子中基因的鉴定及其在群体感应依赖性毒力中的作用特征。