GENETIC DIFFERENTIATION IN AEDES ALBOPICIUS SUBGROUP

白纹伊蚊亚群的遗传分化

• 批准号: 3131576
• 负责人: KARAMJIT S RAI
• 金额: $ 10.51万
• 依托单位: UNIVERSITY OF NOTRE DAME
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-07-01 至 1992-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3131576
• 关键词: Asia; Culicidae; United States; alleles; animal population genetics; biological polymorphism; centromere; chromatin; chromosome aberrations; chromosome inversion; chromosome translocation; chromosomes; communicable disease control; communicable disease transmission; dengue; disease /disorder prevention /control; disease outbreaks; disease vectors; enzymes; gel electrophoresis; gene frequency; genetic crossing over; genetic mapping; genetic strain; genome; geographic site; hybrid cells; karyotype; laboratory mouse; meiosis; mitochondrial DNA; molecular cloning; molecular genetics; nucleic acid hybridization; species difference

Aedes albopictus in an important vector of dengue and dengue hemorrhagic fevers. From its presumed origin in southeast Asia, it has expanded its range from Madagascar to Hawaii. It was introduced into the continental United States last year and rapidly colonized 12 states posing a serious public health threat. The specific aims of the states posing a serious public health threat. The specific aims of the proposed project are to evaluate the vector competence for dengue fever of geographic strains of Aedes albopictus from the U.S. and abroad, determine the origin of U.S. populations and monitor changes in their breeding structure over time, and characterize genetic variation among populations of Aedes albopictus from throughout its range and between five species in the Aedes albopictus subgroup. The vector competence will be evaluated by cataloging variation in susceptibility to oral infection, disseminated infection and transmission among various strains, determining the degree to which these factors are under genetic control (their heritability), and if the heritability is found to be high, selecting for dengue refractory and susceptible strains of Ae. albopictus in order to determine the genetic basis of vector competency. Nuclear DNA amounts, allozymes and restriction maps of mitochondrial DNA will be used to determine origins of U.S. populations and to genetically characterize the same. UPGMA clustering and principal component analysis of DNA amounts and allele frequency data will be used to differentiate populations into subsets. Population specific restriction profiles of mitochondrial DNA will provide definitive information on maternal lineage. Information on allozyme differentiation within and among various cities in the U.S. over the five year period will provide insight into the dynamics of gene frequency changes that accompany colonization by Ae. albopictus. Molecular changes in genome organization and repetitive DNA fraction among various strains of Ae. albopictus and the four other subgroup species will be analysed through CoT curve analysis and cloning of satellite and rapidly reassociating DNA respectively. Chromosomal and reproductive differentiation will be assayed through studies of somatic and meiotic chromosomes and experimental hybridization. The long-term objectives are to utilize the genetic characterization of Ae. albopictus populations and four related species to determine the genetic basis of their vector competence to dengue, to understand the reasons for the rapid spread and to help design effective strategies for the containment/management of the species in U.S.

白纹伊蚊是登革热和登革热的重要传播媒介 出血热。 据推测其起源于东南亚， 它的范围已从马达加斯加扩大到夏威夷。 原来是 去年引入美国大陆并迅速 殖民了 12 个州，对公共卫生构成严重威胁。 这 构成严重公共卫生威胁的国家的具体目标。 拟议项目的具体目标是评估 登革热地理毒株的媒介能力 来自美国和国外的白纹伊蚊，确定来源 美国人口并监测其繁殖变化 随着时间的推移结构，并表征之间的遗传变异 整个分布范围内的白纹伊蚊种群，以及 白纹伊蚊亚群的五个物种之间。 向量能力将通过编目变异来评估 易受口腔感染、播散性感染和 不同菌株之间的传播，确定其程度 这些因素受遗传控制（其遗传力）， 如果发现遗传力较高，则选择登革热 Ae 的难治性和敏感菌株。白纹伊蚊为了 确定载体能力的遗传基础。 核DNA 线粒体 DNA 的量、等位酶和限制性图谱 将用于确定美国人口的起源并 遗传特征相同。 UPGMA 聚类和 DNA 量和等位基因的主成分分析 频率数据将用于将人群区分为 子集。 线粒体的群体特异性限制谱 DNA 将提供有关母系血统的明确信息。 有关各种内部和之间等位酶分化的信息 五年内美国城市将提供见解 伴随而来的基因频率变化的动态 Ae 的殖民化。白纹伊蚊。 基因组的分子变化 不同菌株之间的组织和重复DNA片段 艾。白纹伊蚊和其他四个亚组物种将 通过CoT曲线分析和卫星克隆进行分析 分别快速重新关联DNA。 染色体和 生殖分化将通过研究进行测定 体细胞和减数分裂染色体和实验 杂交。 长期目标是利用基因 Ae 的表征。白纹伊蚊种群和四种相关 物种以确定其载体能力的遗传基础 预防登革热，了解其迅速传播的原因并 帮助设计有效的遏制/管理策略 美国的物种