GENETIC DIFFERENTIATION IN THE AEDES ALBOPICTUS SUBGROUP

白纹伊蚊亚群的遗传分化

• 批准号: 3131573
• 负责人: KARAMJIT S RAI
• 金额: $ 8.78万
• 依托单位: UNIVERSITY OF NOTRE DAME
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-07-01 至 1987-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3131573
• 关键词: Asia; DNA; alleles; animal population genetics; biological polymorphism; centromere; chromatin; chromosome aberrations; chromosome inversion; chromosome translocation; chromosomes; communicable disease transmission; dengue; disease vectors; enzymes; gel electrophoresis; gene frequency; genetic crossing over; genetic mapping; genetic strain; genome; geographic site; hybrid cells; karyotype; meiosis; species difference

The specific aims of the proposed research are to analyze genetic differentiation between 6 species in the Aedes albopictus subgroup and to genetically characterize geographic variation in the widespread species, A. albopictus. The Aedes (Stegomyia) albopictus subgroup consists of 11 species of which A. albopictus is the most important. It is a vector of dengue fever and dengue hemorrhagic fever in southeast Asia. Its wide distribution extends from Madagascar to Hawaii. In recent years it has extended its range to Guam, the south Pacific and has even been reported in light traps in Memphis, TN. Surprisingly, little work has been done on the genetics of this or other related species in the subgroup. The proposed research will help bridge this gap. The long term objectives are to utilize the genetic characterization of A. albopictus populations as well as certain related species in the subgroup to evaluate their potential to serve as efficient vectors of dengue fever. The proposed genetic studies will provide the necessary foundation for future analyses on genetics of dengue transmission and competence of species and strains to transmit the disease. The specific aims, to characterize genetic variation within A. albopictus and between species in the albopictus subgroup, will be accomplished through four different types of analyses. Indirect measures of genetic divergence will be obtained through: 1) experimental hybridization with data collected on insemination, embryonation and egg hatch rates and on the viability of hybrid and backcross progeny, and 2) mate choice tests using species pairs in a series of replicates designed to produce indices of sexual isolation. Direct measures of genetic divergence will be obtained from analyses of chromosome differentiation and electrophoretic detection of enzyme variability. Chromosome differentiation will be examined through a series of studies including Giemsa C-banding to localize heterochromatin, meiotic analysis of hybrids and electrophoretically derived linkage map comparisons to determine the presence of inversions, translocations and deletions or duplications, and cytophotometric analysis of nuclear DNA content. UPGMA clustering and principal component analysis of allele frequency data obtained from allozyme studies will be used to differentiate populations into subsets. If certain diagnostic alleles, loci or discrete allelic frequency differences or chromosomal variants exist among geographic strains of A. albopictus from dengue epidemic versus non-epidemic areas, vector competence may become effectively marked.

这项研究的具体目的是分析遗传 白纹伊蚊亚组6种与 在广泛分布的物种，A. 白纹伊蚊 白纹伊蚊（Stegomyia）亚群由11个 其中A.白纹伊蚊最为重要。 它是一个向量， 东南亚的登革热和登革出血热。 其广泛 分布范围从马达加斯加到夏威夷。 近年来 将其范围扩大到关岛，南太平洋，甚至有报道称， 田纳西州孟菲斯的光陷阱。 令人惊讶的是，在这方面几乎没有做过什么工作。 本种或亚群中其他相关种的遗传学。 拟议 研究将有助于弥合这一差距。 长期目标是利用A. 白纹伊蚊种群以及该亚群中的某些相关物种 以评估它们作为登革热有效媒介的潜力。 拟议的遗传研究将为以下方面提供必要的基础： 登革传播的遗传学和登革病毒感染能力的未来分析 传播疾病的物种和菌株。 本研究的具体目的是研究A.纹伊蚊 以及白纹伊蚊亚组种间的遗传多样性 通过四种不同的分析。 遗传的间接测量 分歧将通过以下方式获得：1）实验杂交， 收集的关于授精、胚胎发育和卵孵化率的数据， 杂种和回交后代的生存力，以及2）使用 物种对在一系列重复设计，以产生指数的 性隔离 遗传分化的直接措施将获得 从染色体分化分析和电泳检测 酶的可变性。 染色体分化将通过 一系列的研究包括Giemsa C-显带定位异染色质， 杂种减数分裂分析和遗传连锁图 比较以确定是否存在倒位、易位和 缺失或重复，以及细胞核DNA的细胞光度分析 内容 等位基因的UPGMA聚类和主成分分析 从等位酶研究中获得的频率数据将用于区分 将人群分成几个子集。 如果某些诊断等位基因，基因座或离散 等位基因频率差异或染色体变异存在于 地理株A.白纹伊蚊与登革热 在非流行区，病媒能力可能变得有效。