GENETIC DIFFERENTIATION IN THE AEDES ALBOPICTUS SUBGROUP

白纹伊蚊亚群的遗传分化

• 批准号: 3131574
• 负责人: KARAMJIT S RAI
• 金额: $ 7.44万
• 依托单位: UNIVERSITY OF NOTRE DAME
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-07-01 至 1987-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3131574
• 关键词: Asia; DNA; alleles; animal population genetics; biological polymorphism; centromere; chromatin; chromosome aberrations; chromosome inversion; chromosome translocation; chromosomes; communicable disease transmission; dengue; disease vectors; enzymes; gel electrophoresis; gene frequency; genetic crossing over; genetic mapping; genetic strain; genome; geographic site; hybrid cells; karyotype; meiosis; species difference

The specific aims of the proposed research are to analyze genetic differentiation between 6 species in the Aedes albopictus subgroup and to genetically characterize geographic variation in the widespread species, A. albopictus. The Aedes (Stegomyia) albopictus subgroup consists of 11 species of which A. albopictus is the most important. It is a vector of dengue fever and dengue hemorrhagic fever in southeast Asia. Its wide distribution extends from Madagascar to Hawaii. In recent years it has extended its range to Guam, the south Pacific and has even been reported in light traps in Memphis, TN. Surprisingly, little work has been done on the genetics of this or other related species in the subgroup. The proposed research will help bridge this gap. The long term objectives are to utilize the genetic characterization of A. albopictus populations as well as certain related species in the subgroup to evaluate their potential to serve as efficient vectors of dengue fever. The proposed genetic studies will provide the necessary foundation for future analyses on genetics of dengue transmission and competence of species and strains to transmit the disease. The specific aims, to characterize genetic variation within A. albopictus and between species in the albopictus subgroup, will be accomplished through four different types of analyses. Indirect measures of genetic divergence will be obtained through: 1) experimental hybridization with data collected on insemination, embryonation and egg hatch rates and on the viability of hybrid and backcross progeny, and 2) mate choice tests using species pairs in a series of replicates designed to produce indices of sexual isolation. Direct measures of genetic divergence will be obtained from analyses of chromosome differentiation and electrophoretic detection of enzyme variability. Chromosome differentiation will be examined through a series of studies including Giemsa C-banding to localize heterochromatin, meiotic analysis of hybrids and electrophoretically derived linkage map comparisons to determine the presence of inversions, translocations and deletions or duplications, and cytophotometric analysis of nuclear DNA content. UPGMA clustering and principal component analysis of allele frequency data obtained from allozyme studies will be used to differentiate populations into subsets. If certain diagnostic alleles, loci or discrete allelic frequency differences or chromosomal variants exist among geographic strains of A. albopictus from dengue epidemic versus non-epidemic areas, vector competence may become effectively marked.

拟议研究的具体目的是分析遗传 白纹伊蚊亚群 6 个物种的区分 遗传表征了广泛分布的物种的地理变异，A. 白纹伊蚊。 白纹伊蚊 (Stegomyia) 亚群由 11 种组成 其中以白纹伊蚊最为重要。 它是一个向量 东南亚的登革热和登革出血热。 其宽 分布范围从马达加斯加到夏威夷。 近年来它已 其活动范围扩大至南太平洋关岛，甚至有报道称 田纳西州孟菲斯的光陷阱。 令人惊讶的是，在这方面几乎没有做任何工作 该亚群中该物种或其他相关物种的遗传学。 拟议的 研究将有助于弥合这一差距。 长期目标是利用 A. 的遗传特征。 白纹伊蚊种群以及该亚群中的某些相关物种 评估它们作为登革热有效载体的潜力。 拟议的遗传学研究将为 登革热传播遗传学和能力的未来分析 传播疾病的物种和菌株。 具体目标是表征白纹伊蚊内的遗传变异 在白纹伊蚊亚群的物种之间，将完成 通过四种不同类型的分析。 遗传的间接测量 分歧将通过以下方式获得：1) 实验杂交 收集有关授精、胚胎和卵孵化率以及 杂交和回交后代的生存能力，以及 2) 使用 一系列重复中的​​物种对，旨在产生指数 性隔离。 将获得遗传差异的直接测量 来自染色体分化和电泳检测的分析 酶的变异性。 染色体分化将通过以下方式进行检查 一系列研究，包括吉姆萨 C 带定位异染色质， 杂种减数分裂分析和电泳衍生连锁图 比较以确定倒位、易位和 核 DNA 的缺失或重复以及细胞光度分析 内容。 等位基因的UPGMA聚类和主成分分析 从等位酶研究中获得的频率数据将用于区分 将群体划分为子集。 如果某些诊断等位基因、基因座或离散 等位基因频率差异或染色体变异存在于 登革热流行病中白纹伊蚊的地理毒株与 在非流行地区，媒介能力可能会得到有效标记。