GENETIC DIFFERENTIATION IN AEDES ALBOPICTUS

白纹伊蚊是登革热和登革热的重要传播媒介 出血热。从它被推定的起源于东南亚， 它已经将范围从马达加斯加扩大到夏威夷。确实是 去年被引入美国大陆，并迅速 殖民了12个州，构成了严重的公共健康威胁。这个 对公众健康构成严重威胁的州的具体目标。 拟议项目的具体目标是评估 登革热地理株的媒介能力 来自美国和国外的白纹伊蚊，确定其来源 并监测它们繁殖的变化 结构随时间的变化，并描述了 白纹伊蚊在整个范围内的种群和 在白纹伊蚊亚群的五种之间。 通过编目变异对载体能力进行评估 易受口腔感染、播散性感染和 在不同的菌株之间传播，决定了 这些因素受基因控制(它们的遗传性)， 如果发现遗传率很高，选择登革热 Ae耐药株和敏感株。白纹伊蚊为了 确定媒介能力的遗传基础。核DNA 线粒体DNA的数量、等位酶和限制性内切酶图谱 将被用来确定美国人口的起源并 在基因上也是如此。UPGMA群集和 DNA含量和等位基因的主成分分析 频率数据将被用来区分人口 子集。线粒体的群体特异性限制性内切酶图谱 DNA将提供有关母系血统的明确信息。 关于等位酶在不同物种内部和之间分化的信息 美国城市在五年期间将提供洞察力 随着基因频率变化的动态变化 Ae.的殖民。白纹伊蚊。基因组中的分子变化 不同菌株间的组织结构和重复DNA片段 天哪。白纹伊蚊和其他四个亚组物种将是 通过COT曲线分析和卫星和克隆分析 分别快速重新关联DNA。染色体和 生殖分化将通过以下研究进行分析 体细胞和减数分裂染色体与实验 杂交。 长期目标是利用基因 Ae.的特征。白纹伊蚊种群及其相关的4个种群 物种决定其媒介能力的遗传基础 登革热，了解迅速传播的原因，并 帮助设计有效的遏制/管理战略 在美国的物种中

Aedes albopictus in an important vector of dengue and dengue hemorrhagic fevers. From its presumed origin in southeast Asia, it has expanded its range from Madagascar to Hawaii. It was introduced into the continental United States last year and rapidly colonized 12 states posing a serious public health threat. The specific aims of the states posing a serious public health threat. The specific aims of the proposed project are to evaluate the vector competence for dengue fever of geographic strains of Aedes albopictus from the U.S. and abroad, determine the origin of U.S. populations and monitor changes in their breeding structure over time, and characterize genetic variation among populations of Aedes albopictus from throughout its range and between five species in the Aedes albopictus subgroup. The vector competence will be evaluated by cataloging variation in susceptibility to oral infection, disseminated infection and transmission among various strains, determining the degree to which these factors are under genetic control (their heritability), and if the heritability is found to be high, selecting for dengue refractory and susceptible strains of Ae. albopictus in order to determine the genetic basis of vector competency. Nuclear DNA amounts, allozymes and restriction maps of mitochondrial DNA will be used to determine origins of U.S. populations and to genetically characterize the same. UPGMA clustering and principal component analysis of DNA amounts and allele frequency data will be used to differentiate populations into subsets. Population specific restriction profiles of mitochondrial DNA will provide definitive information on maternal lineage. Information on allozyme differentiation within and among various cities in the U.S. over the five year period will provide insight into the dynamics of gene frequency changes that accompany colonization by Ae. albopictus. Molecular changes in genome organization and repetitive DNA fraction among various strains of Ae. albopictus and the four other subgroup species will be analysed through CoT curve analysis and cloning of satellite and rapidly reassociating DNA respectively. Chromosomal and reproductive differentiation will be assayed through studies of somatic and meiotic chromosomes and experimental hybridization. The long-term objectives are to utilize the genetic characterization of Ae. albopictus populations and four related species to determine the genetic basis of their vector competence to dengue, to understand the reasons for the rapid spread and to help design effective strategies for the containment/management of the species in U.S.