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GENETIC DIFFERENTIATION IN AEDES ALBOPICIUS SUBGROUP

白纹伊蚊亚群的遗传分化

基本信息

批准号：
3131578
负责人：
KARAMJIT S RAI
金额：
$ 14.31万
依托单位：
UNIVERSITY OF NOTRE DAME
依托单位国家：
美国
项目类别：
财政年份：
1984
资助国家：
美国
起止时间：
1984-07-01 至 1993-02-28
项目状态：
已结题

项目摘要

Aedes albopictus in an important vector of dengue and dengue hemorrhagic fevers. From its presumed origin in southeast Asia, it has expanded its range from Madagascar to Hawaii. It was introduced into the continental United States last year and rapidly colonized 12 states posing a serious public health threat. The specific aims of the states posing a serious public health threat. The specific aims of the proposed project are to evaluate the vector competence for dengue fever of geographic strains of Aedes albopictus from the U.S. and abroad, determine the origin of U.S. populations and monitor changes in their breeding structure over time, and characterize genetic variation among populations of Aedes albopictus from throughout its range and between five species in the Aedes albopictus subgroup. The vector competence will be evaluated by cataloging variation in susceptibility to oral infection, disseminated infection and transmission among various strains, determining the degree to which these factors are under genetic control (their heritability), and if the heritability is found to be high, selecting for dengue refractory and susceptible strains of Ae. albopictus in order to determine the genetic basis of vector competency. Nuclear DNA amounts, allozymes and restriction maps of mitochondrial DNA will be used to determine origins of U.S. populations and to genetically characterize the same. UPGMA clustering and principal component analysis of DNA amounts and allele frequency data will be used to differentiate populations into subsets. Population specific restriction profiles of mitochondrial DNA will provide definitive information on maternal lineage. Information on allozyme differentiation within and among various cities in the U.S. over the five year period will provide insight into the dynamics of gene frequency changes that accompany colonization by Ae. albopictus. Molecular changes in genome organization and repetitive DNA fraction among various strains of Ae. albopictus and the four other subgroup species will be analysed through CoT curve analysis and cloning of satellite and rapidly reassociating DNA respectively. Chromosomal and reproductive differentiation will be assayed through studies of somatic and meiotic chromosomes and experimental hybridization. The long-term objectives are to utilize the genetic characterization of Ae. albopictus populations and four related species to determine the genetic basis of their vector competence to dengue, to understand the reasons for the rapid spread and to help design effective strategies for the containment/management of the species in U.S.

白纹伊蚊是登革热和登革热的重要媒介出血热据推测它起源于东南亚，它的活动范围从马达加斯加扩展到了夏威夷。这是去年被引入美国大陆，殖民了12个州，对公共卫生构成严重威胁。的构成严重公共卫生威胁的国家的具体目标。拟议项目的具体目标是评估登革热地理株的媒介能力白纹伊蚊从美国和国外，确定原产地美国人口的变化，并监测其繁殖随着时间的推移，结构，并表征遗传变异之间白纹伊蚊的种群，白纹伊蚊亚群中的五种之间的差异将通过编目变异来评估载体能力易受口腔感染、播散性感染和各种菌株之间的传播，确定程度，这些因素受遗传控制（遗传力），如果发现遗传性很高，难治性和敏感性菌株。白纹伊蚊，确定病媒能力的遗传基础。核DNA 线粒体DNA的量、等位酶和限制性酶切图谱将用于确定美国人口的起源，基因特征相同。 UPGMA聚类和 DNA含量和等位基因主成分分析频率数据将被用来区分人群，子集线粒体的群体特异性限制性图谱 DNA将提供有关母系血统的明确信息。不同基因型内和不同基因型间的等位酶分化信息在美国的城市在五年期间将提供洞察基因频率变化的动力学，由Ae殖民。白纹伊蚊基因组中的分子变化组织和重复DNA分数之间的各种菌株 AE.白纹伊蚊和其他四个亚种通过CoT曲线分析和卫星克隆分析，快速重新结合DNA。染色体和生殖分化将通过研究体细胞和减数分裂染色体和实验杂交方法长期目标是利用基因 Ae的特性。白纹伊蚊种群和四个相关的物种，以确定其载体能力的遗传基础了解登革热迅速传播的原因，帮助设计有效的遏制/管理战略在美国的物种。