MOLECULAR BIOLOGY OF INVASIVENESS OF CANDIDA ALBICANS

白色念珠菌侵袭性的分子生物学

• 批准号: 3139403
• 负责人: PAUL S SYPHERD
• 金额: $ 14.16万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-09-30 至 1990-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3139403
• 关键词: AIDS; Candida albicans; antifungal antibiotics; candidiasis; cell adhesion; communicable disease control; fungal antigens; fungal genetics; gene expression; genetic markers; genetic regulation; histogenesis; host organism interaction; immunosuppression; laboratory rabbit; microorganism reproduction; molecular cloning; molecular pathology; mutant; nucleic acid probes; opportunistic infections; oral mucosa; protein biosynthesis; radiotracer; virulence

The goal of this research is to provide a molecular basis for understanding the mechanisms for invasiveness of Candida albicans in AIDS patients. The research will start with the relatively clear fact that C. albicans, must adhere to and colonize cells as a stage in establishing an infection. We will use two approaches: (1) a study of mutants that have lost the ability to adhere to animal cells and are subsequently non-pathogenic, and (2) a study of a germ tube-specific protein as a means of understanding the role germ tube penetration plays in invasiveness. We will use gene cloning techniques to study factors that affect the expression of these properties of invasiveness.

这项研究的目标是为以下方面提供分子基础： 了解念珠菌的侵袭机制 艾滋病患者中的白色念珠菌 研究将从 比较明确的事实是C.白色念珠菌，必须粘附并定植 细胞作为建立感染的阶段。 我们将使用两个 方法：（1）研究失去能力的突变体， 粘附于动物细胞，随后为非致病性的，和 (2)一项关于芽管特异性蛋白作为一种手段的研究， 了解芽管穿透在 侵略性 我们将使用基因克隆技术来研究 影响这些侵袭性特性的表达。

项目成果

Both genes for EF-1 alpha in Candida albicans are translated.

白色念珠菌中 EF-1 α 的两个基因均被翻译。

• DOI: 10.1111/j.1365-2958.1991.tb01918.x
• 发表时间: 1991
• 期刊: Molecular microbiology
• 影响因子: 3.6
• 作者: Sundstrom,P;Irwin,M;Smith,D;Sypherd,PS
• 通讯作者: Sypherd,PS

Sequence analysis and expression of the two genes for elongation factor 1 alpha from the dimorphic yeast Candida albicans.

二态性酵母白色念珠菌延伸因子 1 α 的两个基因的序列分析和表达。

• DOI: 10.1128/jb.172.4.2036-2045.1990
• 发表时间: 1990
• 期刊: Journal of bacteriology
• 影响因子: 3.2
• 作者: Sundstrom,P;Smith,D;Sypherd,PS
• 通讯作者: Sypherd,PS