GENE STRUCTURE AND EF-1A IN THE FUNGUS MUCOR

真菌毛霉的基因结构和 EF-1A

• 批准号: 3271993
• 负责人: PAUL S SYPHERD
• 金额: $ 19.6万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-04-01 至 1992-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3271993
• 关键词: developmental genetics; enzyme mechanism; fungal genetics; gene expression; genetic manipulation; genetic transcription; genetic translation; genome; lysine; methylation; molecular cloning; nucleic acid hybridization; nucleic acid sequence; protein biosynthesis; radiotracer; ribosomes; sporogenesis; tissue /cell culture; translation factor

The protein synthesis factor EF-1Alpha of Mucor racemosus hyphae contains 8 or 9 methylated lysines per molecule, while the factor from assexual sporangiospores is non-methylated. During the course of spore germination the specific acitivity increases 6 to 9-fold. This increase in specific activity is accompanied by a constant level of mRNA and a level of EF-1Alpha protein that changes little during the germination period. By isolating and sequencing the EF-1Alpha gene, we found tht it is present in 3 copies in the Mucor genome, each in a different genomic context. This proposal is concerned with two aspects of EF-1Alpha: the role of methylation in "activating" the factor, and the genetic organization, including the control of expression of each gene. We will study the effect of methylation on various EF-1Alpha activities in vitro, locate each methylated site within the protein and purify and study the EF-1Alpha methylases. We will further prepare gene-specific hybridization probes to assess the expression of each gene during several morphogenetic changes (e.g., spore germination, yeast-to-hypha conversions, and sporulation). We also propose to study the 5'flanking regions of each gene to assess the mechanisms controlling transcription.

总状毛霉菌丝蛋白质合成因子EF-1α含8 或每个分子9个甲基化赖氨酸，而来自无性繁殖的因子 孢子囊孢子是非甲基化的。在孢子萌发过程中 比活性提高6~9倍。这一特定数量的增加 活动伴随着恒定水平的信使核糖核酸和一定水平的 EF-1α蛋白，在萌发期变化不大。通过 我们分离并测序了EF-1α基因，发现它存在于 毛霉基因组中有3个拷贝，每个拷贝在不同的基因组环境中。这 该提案涉及EF-1α的两个方面： “激活”因子和遗传组织中的甲基化， 包括对每个基因表达的调控。我们将研究这一影响 在体外各种EF-1α活性上的甲基化，定位每个 蛋白中甲基化位点的表达及EF-1α的纯化和研究 甲基酶。我们将进一步制备基因特异性杂交探针，以 评估每个基因在几种形态发生变化中的表达 (例如，孢子萌发、酵母菌向菌丝转化和产孢子)。我们 还建议研究每个基因的5‘侧翼区，以评估 控制转录的机制。

项目成果

Isolation and sequence analysis of the gene for translation elongation factor 3 from Candida albicans.

• DOI: 10.1093/nar/20.7.1705
• 发表时间: 1992-04
• 期刊: Nucleic acids research
• 影响因子: 14.9
• 作者: K. K. Myers-K.;W. Fonzi;P. Sypherd

Dispersed repetitive DNA sequence of Mucor racemosus.

总状毛霉的分散重复 DNA 序列。

• DOI: 10.1128/jb.162.1.438-440.1985
• 发表时间: 1985
• 期刊: Journal of bacteriology
• 影响因子: 3.2
• 作者: Dewar,R;Katayama,C;Sypherd,PS;Cihlar,RL
• 通讯作者: Cihlar,RL

The isolation and characterization of a calmodulin-encoding gene (CMD1) from the dimorphic fungus Candida albicans.

从二态性真菌白色念珠菌中分离和鉴定钙调蛋白编码基因 (CMD1)。

• DOI: 10.1016/0378-1119(91)90564-r
• 发表时间: 1991
• 期刊: Gene
• 影响因子: 3.5
• 作者: Saporito,SM;Sypherd,PS
• 通讯作者: Sypherd,PS

Structure of a ribosomal protein gene in Mucor racemosus.

总状毛霉核糖体蛋白基因的结构。

• DOI: 10.1093/nar/17.22.9319
• 发表时间: 1989
• 期刊: Nucleic acids research
• 影响因子: 14.9
• 作者: Sosa,L;Fonzi,WA;Sypherd,PS
• 通讯作者: Sypherd,PS

Complementation of the leuB6 allele of Escherichia coli by cloned DNA from Mucor racemosus.

通过从总状毛霉克隆 DNA 来补充大肠杆菌的 leuB6 等位基因。

• DOI: 10.1128/jb.151.1.521-523.1982
• 发表时间: 1982
• 期刊: Journal of bacteriology
• 影响因子: 3.2
• 作者: Cihlar,RL;Sypherd,PS
• 通讯作者: Sypherd,PS