ROLE OF HLA CLASS I MOLECULES IN THE CTL RESPONSE TO HIV

HLA-I类分子在细胞溶解性T细胞的识别中是至关重要的。 淋巴细胞（CTL）。在病毒感染的细胞中， 病毒肽与I类分子结合，并且这种I类/肽复合物 被CTL识别。T细胞在清除几乎所有 病毒感染。在这里提出的实验中，我们将研究 HIV编码肽与MHC I类分子的结合， 结合和CTL识别之间的关系。此外，我们将 对HIV多肽之间的相互作用进行详细分析， 使用肽合成和饱和的组合的I类产品 HLA基因的突变。这些实验将提供重要的 HIV多肽与HLA结合的信息， 用于开发合成疫苗， HLA的变异性

The HLA class I molecules are crucial in the recognition by cytolytic T lymphocytes (CTL) of their targets. In virus infected cells, fragments of viral peptides bind to class I molecules and this class I/peptide complex is recognized by CTLs. T cells are important in the clearance of nearly all virus infections. In the experiments proposed here we will examine the binding of HIV encoded peptides to MHC class I molecules, and the relationship between binding and CTL recognition. In addition, we will undertake a detailed analysis of the interaction between HIV peptides and class I products using a combination of peptide synthesis, and saturation mutagenesis of the HLA genes. These experiments will provide important information on the binding of HIV peptides of HLA and lay the groundwork for the development of synthetic vaccines which can take into account the variability of HLA.