ROLE OF HLA CLASS I MOLECULES IN THE CTL RESPONSE TO HIV

人类白细胞抗原I类分子在细胞溶解T细胞识别中起着至关重要的作用 靶细胞的淋巴细胞(CTL)。在病毒感染的细胞中， 病毒多肽结合到I类分子和这个I类/肽复合体 是CTL识别的。T细胞在清除几乎所有淋巴组织中都很重要。 病毒感染。在这里提出的实验中，我们将检查 HIV编码的多肽与MHC I类分子的结合，以及 结合与CTL识别的关系。此外，我们还将 详细分析艾滋病毒多肽和 I类产品采用多肽合成和饱和相结合的方式 人类白细胞抗原基因的诱变。这些实验将提供重要的 了解人类免疫缺陷病毒与人类白细胞抗原的结合情况，为下一步的研究奠定基础 用于开发合成疫苗，这种疫苗可以考虑到 人类白细胞抗原的变异性。

The HLA class I molecules are crucial in the recognition by cytolytic T lymphocytes (CTL) of their targets. In virus infected cells, fragments of viral peptides bind to class I molecules and this class I/peptide complex is recognized by CTLs. T cells are important in the clearance of nearly all virus infections. In the experiments proposed here we will examine the binding of HIV encoded peptides to MHC class I molecules, and the relationship between binding and CTL recognition. In addition, we will undertake a detailed analysis of the interaction between HIV peptides and class I products using a combination of peptide synthesis, and saturation mutagenesis of the HLA genes. These experiments will provide important information on the binding of HIV peptides of HLA and lay the groundwork for the development of synthetic vaccines which can take into account the variability of HLA.