WATER AND ELECTROLYTE TRANSPORT BY THE INTESTINE

安装在通量室中的兔回肠的电场刺激（EFS） 增加了腔电负性，增加了短路 电流，降低组织总离子电导，并导致氯化物分泌。 类似的变化可能是由蝎子毒液（Leiurus quinquestriatus）引起的， 使神经去极化;对EFS的反应可以通过药物来预防， 干扰神经传递，即，蝎毒，藜芦碱，50 mM K， 河豚毒素 化学传递者的身份尚不清楚，但它是 很明显，这种反应需要沐浴介质中的钙。 我们计划研究 以确定：1）通过确定是否存在化学传递物来确定化学传递物的身份。 可以用血管活性肠肽抗体预防反应，或 2）细胞旁分流在介导P物质分泌中的作用 EFS引起的变化; 3）EFS对其他上皮细胞的影响 消化道，包括从结肠和小肠获得的消化道， 被切除组织的病人。

Electrical field stimulation (EFS) of rabbit ileum mounted in a flux chamber increases the luminal electrical negativity, increases the short circuit current, reduces tissue total ionic conductance, and causes chloride secretion. Similar changes may be caused by scorpion venom (Leiurus quinquestriatus) which depolarizes nerves; the responses to EFS may be prevented by agents which interfere with neural transmission, viz, scorpion venom, veratrine, 50 mM K, and tetrodotoxin. The identity of the chemical transmitter is not known, but it is clear that the response requires calcium in the bathing medium. We plan studies to determine: 1) the identity of the chemical transmitter by determining if the response can be prevented with antibodies to Vasoactive Intestinal Peptide, or to Substance P; 2) the role of the paracellular shunt in mediating the secretory changes caused by EFS; and 3) the effects of EFS on other epithelia of the alimentary canal, including that obtained from the colon and small intestine of patients from whom the tissues are being resected.