WATER AND ELECTROLYTE TRANSPORT BY THE INTESTINE

肠道的水和电解质运输

• 批准号: 3151158
• 负责人: KENNETH A. HUBEL
• 金额: $ 10.66万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-07-01 至 1987-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3151158
• 关键词: acetylcholine; autonomic nervous system; cholinergic agents; electrolyte balance; electrostimulus; gastrointestinal absorption /transport; innervation; intestinal mucosa; neurotoxins; neurotransmitters; tetrodotoxin

Electrical field stimulation (EFS) of rabbit ileum mounted in a flux chamber increases the luminal electrical negativity, increases the short circuit current, reduces tissue total ionic conductance, and causes chloride secretion. Similar changes may be caused by scorpion venom (Leiurus quinquestriatus) which depolarizes nerves; the responses to EFS may be prevented by agents which interfere with neural transmission, viz, scorpion venom, veratrine, 50 mM K, and tetrodotoxin. The identity of the chemical transmitter is not known, but it is clear that the response requires calcium in the bathing medium. We plan studies to determine: 1) the identity of the chemical transmitter by determining if the response can be prevented with antibodies to Vasoactive Intestinal Peptide, or to Substance P; 2) the role of the paracellular shunt in mediating the secretory changes caused by EFS; and 3) the effects of EFS on other epithelia of the alimentary canal, including that obtained from the colon and small intestine of patients from whom the tissues are being resected.

安装在通量室中的兔回肠的电场刺激（EFS） 增加了腔电负性，增加了短路 电流，降低组织总离子电导，并导致氯化物分泌。 类似的变化可能是由蝎子毒液（Leiurus quinquestriatus）引起的， 使神经去极化;对EFS的反应可以通过药物来预防， 干扰神经传递，即，蝎毒，藜芦碱，50 mM K， 河豚毒素 化学传递者的身份尚不清楚，但它是 很明显，这种反应需要沐浴介质中的钙。 我们计划研究 以确定：1）通过确定是否存在 可以用血管活性肠肽抗体预防反应，或 2）细胞旁分流在介导P物质分泌中的作用 EFS引起的变化; 3）EFS对其他上皮细胞的影响 消化道，包括从结肠和小肠获得的消化道， 被切除组织的病人。

项目成果

Human ileal ion transport in vitro: changes with electrical field stimulation and tetrodotoxin.

体外人回肠离子转运：电场刺激和河豚毒素的变化。

• 发表时间: 1982
• 期刊: Gastroenterology
• 影响因子: 29.4
• 作者: Hubel,KA;Shirazi,S
• 通讯作者: Shirazi,S

Effects of scorpion venom on electrolyte transport by rabbit ileum.

蝎毒对兔回肠电解质转运的影响。

• DOI: 10.1152/ajpgi.1983.244.5.g501
• 发表时间: 1983
• 期刊: The American journal of physiology
• 作者: Hubel,KA

Effect of veratrine and 50 mM K on ileal transport and electrically induced secretion.

藜芦碱和 50 mM K 对回肠运输和电诱导分泌的影响。

• DOI: 10.1152/ajpgi.1981.240.3.g211
• 发表时间: 1981
• 期刊: The American journal of physiology
• 作者: Hubel,KA

Electrical stimulus-secretion coupling in rabbit ileal mucosa.

兔回肠粘膜的电刺激-分泌耦合。

• 发表时间: 1984
• 期刊: The Journal of pharmacology and experimental therapeutics
• 作者: Hubel,KA

Effect of neuropeptide Y on ion transport by the rabbit ileum.

神经肽 Y 对兔回肠离子转运的影响。

• 发表时间: 1986
• 期刊: The Journal of pharmacology and experimental therapeutics
• 作者: Hubel,KA;Renquist,KS
• 通讯作者: Renquist,KS