NEUROCHEMISTRY OF THE DIENCEPHALON

拟议研究的目标是继续调查 下丘脑多肽在调节血管紧张素转换酶分泌中的作用 脑下垂体前叶和其他身体功能的控制。这个 这项工作的目标包括： 1.从猪下丘脑组织中分离纯化 催乳素释放抑制激素 (PRH)，一种抗分裂(生长抑制)因子，更大，更基本 CRF和其他具有生物活性的多肽的形式。 2.这些多肽的结构测定。 3.这些神经激素及其类似物的合成。某些多肽 还将合成片段以确定其特异性 我们的放射免疫分析适用于CRF和GH-RF。 4.中华绒毛虫的生物学、化学和免疫学特性比较 猪下丘脑激素与其他物种激素的比较及定位 结构差异和构效关系的测定 下丘脑各种激素之间的关系。 5.这些多肽的抗血清的制备 放射免疫分析和免疫细胞化学定位研究。 6.对天然下丘脑的广泛生理生化研究 激素、合成激素及其合成类似物，包括 半衰期、代谢和失活的研究。这些研究 对于规划现实的临床试验将是重要的。一项调查 慢性应用下丘脑激素对某些疾病的影响 内分泌依赖型或激素敏感型肿瘤也将进行治疗。 7.继续寻找促卵泡激素释放激素 (FSH-RH)有别于十肽LH-RH(Gn-RH)。 8.下丘脑肽对血脂影响的实验研究 运动，食欲和胃酸分泌；纯化，分离， 对其中一些多肽的结构和合成的测定。 9.向临床医生提供有关自然和精神疾病的详细信息和想法 用于计划人体临床试验的合成材料。

The objective of the proposed research is the continued investigation of the role the hypothalamic peptides play in the regulation of secretion of the anterior pituitary gland and in control of other bodily functions. The goals of this work include: 1. The isolation from porcine hypothalamic tissue of Prolactin-Release-Inhibiting Hormone (PIF), Prolactin Releasing Hormone (PRH), an antimitogenic (growth inhibiting) factor, larger and more basic forms of CRF and other peptides with biological activities. 2. The determination of structure of these peptides. 3. The synthesis of these neurohormones and their analogs. Some peptide fragments will be also synthesized in order to determine the specificity of our RIA's for CRF and GH-RF. 4. Comparison of biological, chemical and immunological properties of porcine hypothalamic hormones with those of other species and location of structural differences, and determination of structure-activity relationship for various hypothalamic hormones. 5. Generation of antisera to these peptides for the development of radioimmunoassays and immunocytochemical localization studies. 6. Extensive physiological and biochemical studies on natural hypothalamic hormones, synthetic hormones and their synthetic analogs, including investigation of half life, metabolism and inactivation. These studies will be important for planning realistic clinical tests. An investigation of the effects of chronic administration of hypothalamic hormones on some endocrine dependent or hormone sensitive tumors will be also carried out. 7. Continued search for a follicle stimulating hormone-releasing hormone (FSH-RH) distinct from the decapeptide LH-RH (Gn-RH). 8. The investigation of the effect of hypothalamic peptides on lipid mobilization, appetite and gastric acid secretion; purification, isolation, determination of structure and synthesis of some of these peptides. 9. Providing of detailed information and ideas to clinicians on natural and synthetic materials for the planning of clinical tests on human subjects.