Pan-genome reverse vaccinology of Staphylococcus aureus bovine mastitis

金黄色葡萄球菌牛乳腺炎的全基因组逆向疫苗学

• 批准号: BB/K00638X/1
• 负责人: Ross Fitzgerald
• 金额: $ 41.37万
• 依托单位: University of Edinburgh
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2013
• 资助国家: 英国
• 起止时间: 2013 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FK00638X%2F1
• 关键词: Pan; genome; reverse; vaccinology; Staphylococcus

Staphylococcus aureus is an important cause of hospital- and community-associated infections of humans. In addition, S. aureus causes economically-important infections of livestock such as bovine mastitis (infection of the udder) which is a major economic burden on the global dairy industry and an important animal welfare issue. Bovine mastitis is typically difficult to treat with antibiotics due in part to the capacity of S. aureus to become intracellular, and antibiotic-resistance is an increasing problem. Accordingly, a vaccine which protects against S. aureus mastitis would be highly desirable. Recently, the availability of the entire genetic code for pathogenic bacteria has been has been useful for identification of antigens predicted to induce a protective immune response. Such studies have resulting in promising vaccines for several important human pathogens. Here, we propose to utilise a similar approach based on the genetic code for all of the subtypes of S. aureus which cause bovine mastitis. This will allow us to identify antigens which are made by all strains, and which induce antibodies which promote bacterial killing and disrupt host-pathogen interactions. Importantly, the capacity to induce protective immunity will be examined in dairy cows. Overall, the study will result in a better understanding of how S. aureus causes bovine mastitis and which antigens may be useful as a vaccine to prevent disease, increase productivity and reduce animal suffering.

金黄色葡萄球菌是人类医院和社区相关感染的重要原因。此外，S.金黄色葡萄球菌引起家畜的经济上重要的感染，例如牛乳腺炎（乳房感染），这是全球乳制品工业的主要经济负担和重要的动物福利问题。牛乳腺炎通常难以用抗生素治疗，部分原因是由于S。金黄色葡萄球菌变成细胞内的，并且耐药性是一个日益严重的问题。因此，保护免受S.金黄色乳腺炎是非常理想的。最近，病原菌的整个遗传密码的可用性已被用于鉴定预测诱导保护性免疫应答的抗原。这些研究已经产生了针对几种重要的人类病原体的有希望的疫苗。在这里，我们建议利用一个类似的方法的基础上的遗传密码的所有亚型的S。金黄色葡萄球菌引起牛乳腺炎。这将使我们能够识别由所有菌株产生的抗原，并诱导促进细菌杀伤和破坏宿主-病原体相互作用的抗体。重要的是，将在奶牛中检查诱导保护性免疫的能力。总的来说，这项研究将导致更好地了解S。金黄色葡萄球菌会导致牛乳腺炎，这些抗原可能可用作疫苗来预防疾病、提高生产力并减少动物痛苦。

项目成果

Identification of LukPQ, a novel, equid-adapted leukocidin of Staphylococcus aureus.

LUKPQ的鉴定，这是金黄色葡萄球菌的新型，均等的白细胞素。

• DOI: 10.1038/srep40660
• 发表时间: 2017-01-20
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Koop G;Vrieling M;Storisteanu DM;Lok LS;Monie T;van Wigcheren G;Raisen C;Ba X;Gleadall N;Hadjirin N;Timmerman AJ;Wagenaar JA;Klunder HM;Fitzgerald JR;Zadoks R;Paterson GK;Torres C;Waller AS;Loeffler A;Loncaric I;Hoet AE;Bergström K;De Martino L;Pomba C;de Lencastre H;Ben Slama K;Gharsa H;Richardson EJ;Chilvers ER;de Haas C;van Kessel K;van Strijp JA;Harrison EM;Holmes MA
• 通讯作者: Holmes MA

Recombination-Mediated Host Adaptation by Avian Staphylococcus aureus.

• DOI: 10.1093/gbe/evx037
• 发表时间: 2017-04-01
• 期刊: Genome biology and evolution
• 影响因子: 3.3
• 作者: Murray S;Pascoe B;Méric G;Mageiros L;Yahara K;Hitchings MD;Friedmann Y;Wilkinson TS;Gormley FJ;Mack D;Bray JE;Lamble S;Bowden R;Jolley KA;Maiden MCJ;Wendlandt S;Schwarz S;Corander J;Fitzgerald JR;Sheppard SK
• 通讯作者: Sheppard SK

Phage-mediated horizontal transfer of a Staphylococcus aureus virulence-associated genomic island.

• DOI: 10.1038/srep09784
• 发表时间: 2015-04-20
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Moon BY;Park JY;Hwang SY;Robinson DA;Thomas JC;Fitzgerald JR;Park YH;Seo KS
• 通讯作者: Seo KS

Evolutionary and Functional Analysis of Coagulase Positivity among the Staphylococci.

• DOI: 10.1128/msphere.00381-21
• 发表时间: 2021-08-25
• 期刊: mSphere
• 影响因子: 4.8
• 作者: Pickering AC;Yebra G;Gong X;Goncheva MI;Wee BA;MacFadyen AC;Muehlbauer LF;Alves J;Cartwright RA;Paterson GK;Fitzgerald JR
• 通讯作者: Fitzgerald JR