Genomic analysis of the host-specific pathogenesis of Staphylococcus aureus

金黄色葡萄球菌宿主特异性发病机制的基因组分析

• 批准号: BB/D521222/1
• 负责人: Ross Fitzgerald
• 金额: $ 29.66万
• 依托单位: University of Edinburgh
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2006
• 资助国家: 英国
• 起止时间: 2006 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FD521222%2F1
• 关键词: Genomic; analysis; host; specific; pathogenesis

Staphylococus aureus causes life-threatening infections of humans and economically important infections of domestic animals such as mastitis. There is very limited cross-species transfer of infections suggesting that individual S. aureus clones have evolved the mechanisms required to infect a single host only. The purpose of this project is to identify what factors are produced by S. aureus which contribute to pathogenesis of specific animal hosts. In addition, the evolution of S. aureus clones infecting different animal species will be explored. The work will be carried out using whole-genome microarrays. Microarrays consist of glass slides which can represent each of the genes identified in the whole genome of that organism. The microarray allows the comparison of the genome content of many bacterial isolates. We will then determine if the genes are being expressed during animal infection by measuring the antibody response to specific factors. This would indicate that the proteins are involved in host-pathogen interactions and may play a role in causing disease. Taken together, this study will provide new insights into S. aureus evolution and pathogenesis and will identify new targets for vaccine or therapeutic development.

金黄色葡萄球菌引起危及生命的人类感染和经济上重要的家畜感染，如乳腺炎。感染的跨物种转移非常有限，这表明单个金黄色葡萄球菌克隆已经进化出仅感染单一宿主所需的机制。本项目的目的是确定金黄色葡萄球菌产生的哪些因素有助于特定动物宿主的发病机制。此外，还将探讨金黄色葡萄球菌克隆感染不同动物物种的进化过程。这项工作将使用全基因组微阵列进行。微阵列由玻片组成，玻片可以代表该生物体整个基因组中识别的每个基因。微阵列允许比较许多细菌分离物的基因组内容。然后，我们将通过测量抗体对特定因素的反应来确定这些基因是否在动物感染期间被表达。这表明这些蛋白参与了宿主与病原体的相互作用，并可能在致病过程中发挥作用。总之，这项研究将为金黄色葡萄球菌的进化和发病机制提供新的见解，并将确定疫苗或治疗开发的新靶点。

项目成果

Molecular basis of virulence in Staphylococcus aureus mastitis.

• DOI: 10.1371/journal.pone.0027354
• 发表时间: 2011
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Le Maréchal C;Seyffert N;Jardin J;Hernandez D;Jan G;Rault L;Azevedo V;François P;Schrenzel J;van de Guchte M;Even S;Berkova N;Thiéry R;Fitzgerald JR;Vautor E;Le Loir Y
• 通讯作者: Le Loir Y

Evolutionary genomics of Staphylococcus aureus reveals insights into the origin and molecular basis of ruminant host adaptation.

• DOI: 10.1093/gbe/evq031
• 发表时间: 2010-07-12
• 期刊: Genome biology and evolution
• 影响因子: 3.3
• 作者: Guinane CM;Ben Zakour NL;Tormo-Mas MA;Weinert LA;Lowder BV;Cartwright RA;Smyth DS;Smyth CJ;Lindsay JA;Gould KA;Witney A;Hinds J;Bollback JP;Rambaut A;Penadés JR;Fitzgerald JR
• 通讯作者: Fitzgerald JR

Human origin for avian pathogenic Staphylococcus aureus.

人类起源为禽类致病性金黄色葡萄球菌。

• DOI: 10.4161/viru.1.4.11838
• 发表时间: 2010
• 期刊: Virulence
• 影响因子: 5.2
• 作者: Lowder BV

Evolutionary genomics of Staphylococcus aureus reveals insights into the origin and molecular basis of ruminant host adaptation

金黄色葡萄球菌的进化基因组学揭示了反刍动物宿主适应的起源和分子基础

• DOI: 10.17863/cam.8994
• 发表时间: 2010
• 作者: Guinane C