ROLE OF CELL SURFACE IN INITIATION OF CELL DIVISION
细胞表面在细胞分裂起始中的作用
基本信息
- 批准号:3163623
- 负责人:
- 金额:$ 16.16万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1979
- 资助国家:美国
- 起止时间:1979-06-01 至 1989-05-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Our studies on the mechanism by which thrombin initiates division of
cultured fibroblasts have shown that action at the cell surface is
sufficient for cell activation. This prompted studies on the cell surface
for interactions and events necessary for the stimulation of cell division.
These studies led to the identification of protease nexin, a cell-secreted
protein which mediates much of the specific binding of thrombin to the cell
surface. Protease nexin is released by cells into the culture medium where
it forms a covalent linkage with thrombin. The thrombin-protease nexin
complexes then specifically bind to cells and are internalized and
degraded. Our studies have shown that linkage of thrombin to protease
nexin inactivates the thrombin. Moreover, protease nexin inhibits the
stimulation by thrombin, and thus it represents a mechanism by which cells
modulate their mitogenic response to thrombin. We have also demonstrated a
cell surface binding site for unlinked thrombin and have shown that binding
of thrombin to this site is not necessary for the stimulation of cell
division. Past studies have shown that the proteolytic activity of
thrombin is necessary for cell activation. In fact, all of our results
point to a requirement for cleavage of one or more cell surface proteins by
thrombin for the stimulation. At\this point we have shown that several cell
surface proteins are cleaved by thrombin. One of these has been identified
as fibronectin. In addition, cell surface proteins of about 140
kilodaltons and 55 kilodaltons were thrombin-sensitive, but they have not
yet been identified. Studies\are underway to better resolve membrane
proteins so we will be able to identify additional ones that might be
thrombin-sensitive. We will study which of these cleavages are necessary
for cell activation by determining whether they occur in a large series of
cloned cell populations that are either responsive or unresponsive to the
mitogenic action of thrombin. (A)
凝血酶启动细胞分裂机制的研究
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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DENNIS D CUNNINGHAM其他文献
DENNIS D CUNNINGHAM的其他文献
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{{ truncateString('DENNIS D CUNNINGHAM', 18)}}的其他基金
Ventilated Mouse Cages for NIH Funded Construction Projects
用于 NIH 资助的建设项目的通风鼠笼
- 批准号:
7086571 - 财政年份:2006
- 资助金额:
$ 16.16万 - 项目类别:
Extramural Research Facilities Construction Breast & Wo*
校外研究设施建设乳房
- 批准号:
6984001 - 财政年份:2005
- 资助金额:
$ 16.16万 - 项目类别:
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