IMMUNE EFFECTOR MECHANISMS IN EBV CARRYING PATIENTS

这个项目研究了EBV感染细胞和 正常人和免疫缺陷者的宿主免疫系统。对于一个 提高对这一复杂系统的认识，有必要拓宽 我们对病毒与不同宿主相互作用的认识 细胞在体外和体内，以及病毒之间的相互作用 被感染的细胞和宿主。本项目旨在分析 两个层面上的某些问题，以相互关联的方式。 该项目由以下部分组成： 一、EBV和EBV携带细胞的免疫应答 实验动物模型。 病毒-细胞-宿主在正常和正常状态下体内相互作用 病理情况。 三、临床相关研究。 第一部分将讨论细胞反应的激活。 自体和异体系统，EBV特异性的鉴定 拒绝目标，验证或证伪我们的假设的实验 不同生长转化的判别T细胞识别 EBV相关蛋白及其表型依赖性差异的研究 B细胞对免疫排斥反应的敏感性。 第二部分将讨论EBV基因组的存在和表达。 体内不同B细胞亚群与EBV相关的进化 恶性上皮细胞和B细胞表型。 第三部分将研究对病毒的体液和细胞反应。 健康血清阳性者、单核细胞增多症患者和 骨髓移植中的免疫缺陷者、病毒种群交换 患者，免疫缺陷者中携带EBV的免疫母细胞增殖， EB病毒在携带慢性淋巴细胞病毒亚组分中的作用 白血病细胞，以及EBV在鼻咽癌中的作用。

This project deals with the interactions between EBV-infected cells and the host immune system in normal and immunodefective persons. For an improved understanding of this complex system, it is necessary to broaden our knowledge both on the interaction of the virus with different host cells in vitro and in vivo, and on the interplay between the virally infected cells and the host. This project aims at the analysis of certain questions at both levels, in a mutually interrelated way. The project consists of the following parts: I.Immune responses to EBV and EBV-carrying cells in vitro and in an experimental animal model. II.Virus-cell-host interactions in vivo under normal and pathological conditions. III.Clinically related studies. Part I will deal with the activation of the cellular responses in autologous and allogeneic systems, identification of the EBV-specific rejection targets, experiments to verify or falsify our hypotheses of discriminatory T-cell recognition of different growth transformation associated EBV-proteins, and the study of phenotype dependent differences in B-cell sensitivity to immune rejection. Part II will deal with the presence and expression of EBV genomes in different B-cell subpopulations in vivo, and the evolution of EBV-related malignant epithelial and B-cell phenotypes. Part III will study humoral and cellular responses to virally encoded antigens in healthy seropositives, mononucleosis patients and immunodefectives, viral population exchange in bone marrow transplant patients, EBV-carrying immunoblastic proliferations in immunodefectives, the role of EBV in subfractions of virus carrying chronic lymphocytic leukemia cells, and the role of EBV in nasopharyngeal carcinoma.