EB-VIRAL IN LATENCY--TRANSFORMATION AND IMMUNE ESCAPE

潜伏期的 EB 病毒——转化和免疫逃逸

• 批准号: 3197033
• 负责人: GEORGE KLEIN
• 金额: $ 9.84万
• 依托单位: KAROLINSKA INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-07-01 至 1993-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3197033
• 关键词: B lymphocyte; Burkitt's lymphoma; Epstein Barr virus; complementary DNA; epithelioma; gene expression; genetic manipulation; genetic promoter element; genetic transcription; human tissue; immunity; latent virus infection; lymphoblast; molecular cloning; nasopharyngeal neoplasms; oncogenes; polymerase chain reaction; viral leukemogenesis; virus antigen; virus genetics

The broad objective of this study is to elucidate the strategy of the Epstein-Barr virus (EBV), particularly as it relates to the persistence of the virus, unrecognized by the immune system, during the entire lifetime of seropositive individuals. Our findings suggest that small heavy B- lymphocytes may harbor the latent viral genome. We shall study the expression of the viral genome in these cells, in comparison with its malignant counterpart, Burkitt's lymphoma, and in contrast to the acutely infected, proliferating blast population in vitro (LCL) and in vivo (immunoblastic EBV-carrying lymphomas in immuno-defectives). The findings are expected to elucidate the regulation of viral expression, the immunogenicity of different viral products, and the occurrence of cell phenotype dependent modulations n viral transcription. Considered together with our parallel work on the Ig/myc translocation, this work will also contribute to the understanding of Burkitt lymphoma development. A special subproject concerns the interactions of the virus with epithelial cells, in view of its documented association with nasopharyngeal carcinoma.

本研究的主要目标是阐明 EB病毒（EBV），特别是因为它涉及持久性的 在整个生命周期中，免疫系统无法识别病毒， 血清阳性个体。 我们的研究结果表明，小而重的B- 淋巴细胞可能携带潜伏的病毒基因组。 我们将研究 病毒基因组在这些细胞中的表达，与其 恶性对应物伯基特淋巴瘤，与急性 体外和体内感染的增殖母细胞群 （免疫缺陷者中的免疫母细胞性携带EBV的淋巴瘤）。 这些发现 有望阐明病毒表达的调节， 不同病毒产物的免疫原性，以及细胞 表型依赖性调节病毒转录。 一起考虑 与我们对IG/myc易位的平行工作一样，这项工作也将 有助于了解伯基特淋巴瘤的发展。 一个特殊 子项目涉及病毒与上皮细胞的相互作用， 鉴于其与鼻咽癌的相关性。

项目成果

Co-localization of the retinoblastoma protein and the Epstein-Barr virus-encoded nuclear antigen EBNA-5.

视网膜母细胞瘤蛋白和 Epstein-Barr 病毒编码的核抗原 EBNA-5 共定位。

• DOI: 10.1016/0014-4827(91)90438-z
• 发表时间: 1991
• 期刊: Experimental cell research
• 影响因子: 3.7
• 作者: Jiang,WQ;Szekely,L;Wendel-Hansen,V;Ringertz,N;Klein,G;Rosén,A
• 通讯作者: Rosén,A

Cell phenotype (CD23)-dependent variation in EBV genome copy numbers within lymphoblastoid cell lines (LCL).

淋巴母细胞系 (LCL) 内 EBV 基因组拷贝数的细胞表型 (CD23) 依赖性变异。

• DOI: 10.1002/ijc.2910500417
• 发表时间: 1992
• 期刊: International journal of cancer
• 影响因子: 6.4
• 作者: Wendel-Hansen,V;Tao,W;Ericson,M;Klein,G;Rosén,A
• 通讯作者: Rosén,A

Development of a recombinant enzyme-linked immunosorbent assay for detection of antibodies against Epstein-Barr virus nuclear antigens 2A and 2B.

开发重组酶联免疫吸附测定法，用于检测 Epstein-Barr 病毒核抗原 2A 和 2B 的抗体。

• DOI: 10.1128/jcm.32.1.112-120.1994
• 发表时间: 1994
• 期刊: Journal of clinical microbiology
• 影响因子: 9.4
• 作者: Geertsen,R;Espander-Jansson,A;Dobec,M;Price,P;Wunderli,W;Rymo,L
• 通讯作者: Rymo,L

Methylation of discrete sites within the enhancer region regulates the activity of the Epstein-Barr virus BamHI W promoter in Burkitt lymphoma lines.

增强子区域内离散位点的甲基化调节伯基特淋巴瘤系中 Epstein-Barr 病毒 BamHI W 启动子的活性。

• DOI: 10.1128/jvi.66.1.62-69.1992
• 发表时间: 1992
• 期刊: Journal of virology
• 影响因子: 5.4
• 作者: Jansson,A;Masucci,M;Rymo,L
• 通讯作者: Rymo,L

Viral and cellular factors influence the activity of the Epstein-Barr virus BCR2 and BWR1 promoters in cells of different phenotype.

病毒和细胞因素影响不同表型细胞中 Epstein-Barr 病毒 BCR2 和 BWR1 启动子的活性。

• DOI: 10.1006/viro.1993.1186
• 发表时间: 1993
• 期刊: Virology
• 影响因子: 3.7
• 作者: Nilsson,T;Sjoblom,A;Masucci,MG;Rymo,L
• 通讯作者: Rymo,L