IMMUNE EFFECTOR MECHANISMS IN EBV CARRYING PATIENTS

EBV 携带患者的免疫效应机制

• 批准号: 3169170
• 负责人: GEORGE KLEIN
• 金额: $ 12.65万
• 依托单位: KAROLINSKA INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1981
• 资助国家: 美国
• 起止时间: 1981-09-01 至 1994-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3169170
• 关键词: B lymphocyte; Epstein Barr virus; athymic mouse; bone marrow transplantation; cellular immunity; disease /disorder model; human subject; immunodeficiency; laboratory mouse; latent virus infection; lymphocytic leukemia; mononucleosis; nasopharyngeal neoplasms; viral carcinogenesis; virus antigen; virus protein

This project deals with the interactions between EBV-infected cells and the host immune system in normal and immunodefective persons. For an improved understanding of this complex system, it is necessary to broaden our knowledge both on the interaction of the virus with different host cells in vitro and in vivo, and on the interplay between the virally infected cells and the host. This project aims at the analysis of certain questions at both levels, in a mutually interrelated way. The project consists of the following parts: I.Immune responses to EBV and EBV-carrying cells in vitro and in an experimental animal model. II.Virus-cell-host interactions in vivo under normal and pathological conditions. III.Clinically related studies. Part I will deal with the activation of the cellular responses in autologous and allogeneic systems, identification of the EBV-specific rejection targets, experiments to verify or falsify our hypotheses of discriminatory T-cell recognition of different growth transformation associated EBV-proteins, and the study of phenotype dependent differences in B-cell sensitivity to immune rejection. Part II will deal with the presence and expression of EBV genomes in different B-cell subpopulations in vivo, and the evolution of EBV-related malignant epithelial and B-cell phenotypes. Part III will study humoral and cellular responses to virally encoded antigens in healthy seropositives, mononucleosis patients and immunodefectives, viral population exchange in bone marrow transplant patients, EBV-carrying immunoblastic proliferations in immunodefectives, the role of EBV in subfractions of virus carrying chronic lymphocytic leukemia cells, and the role of EBV in nasopharyngeal carcinoma.

该项目涉及EB病毒感染细胞与 正常人和免疫缺陷者的宿主免疫系统。 用于 为了更好地了解这个复杂的系统，有必要扩大 我们对病毒与不同宿主相互作用的了解 细胞在体外和体内，并在病毒之间的相互作用， 感染的细胞和宿主 本项目旨在分析 在这两个层面上的某些问题，以相互关联的方式。 该项目由以下部分组成： I.在体外和体内对EBV和携带EBV的细胞的免疫应答 实验动物模型 II.正常和非正常条件下病毒-细胞-宿主的体内相互作用 病理条件。 III.临床相关研究。 第一部分将处理激活的细胞反应， 自体和同种异体系统，EBV特异性 拒绝目标，实验来验证或证伪我们的假设， 不同生长转化的区分性T细胞识别 相关的EBV蛋白，以及表型依赖性差异的研究 对免疫排斥反应的敏感性 第二部分将处理EB病毒基因组的存在和表达， 体内不同B细胞亚群，以及EBV相关的 恶性上皮和B细胞表型。 第三部分将研究体液和细胞对病毒的反应。 健康血清阳性、单核细胞增多症患者和 免疫缺陷者，骨髓移植中病毒群体交换 患者，免疫缺陷者中携带EBV的免疫母细胞增殖， EB病毒在携带慢性淋巴细胞性白血病病毒亚群中作用 白血病细胞，以及EBV在鼻咽癌中的作用。

项目成果

Serum anti-EBV IgA is inversely related to tumor infiltration by surface IgA positive lymphocytes in nasopharyngeal carcinoma.

鼻咽癌血清抗EBV IgA与表面IgA阳性淋巴细胞的肿瘤浸润呈负相关。

• 发表时间: 1983
• 期刊: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
• 作者: Gunven,P;Klein,G;Henle,W;Henle,G;Forsby,N
• 通讯作者: Forsby,N

Epstein-Barr virus infection in allogeneic marrow grafting: lessons for transplant physicians and virologists.

同种异体骨髓移植中的 Epstein-Barr 病毒感染：移植医生和病毒学家的教训。

• DOI: 10.1007/bf01715373
• 发表时间: 1992
• 期刊: Annals of hematology
• 影响因子: 3.5
• 作者: Gratama,JW;Oosterveer,MA;Lepoutre,J;Fibbe,WE;Ringdén,O;Vossen,JM;Willemze,R;Bolhuis,RL;vanRood,JJ;Ernberg,I
• 通讯作者: Ernberg,I

Tumorigenicity of EBV-carrying lymphoblastoid cell lines (LCLs): distinctive grading in SCID mice.

携带 EBV 的淋巴母细胞系 (LCL) 的致瘤性：SCID 小鼠的独特分级。

• DOI: 10.1002/ijc.2910490420
• 发表时间: 1991
• 期刊: International journal of cancer
• 影响因子: 6.4
• 作者: Ramqvist,T;Norén,L;Iwarsson,K;Klein,G
• 通讯作者: Klein,G

Clonality and methylation status of the Epstein-Barr virus (EBV) genomes in in vivo-infected EBV-carrying chronic lymphocytic leukemia (CLL) cell lines.

体内感染的携带 EBV 的慢性淋巴细胞白血病 (CLL) 细胞系中 Epstein-Barr 病毒 (EBV) 基因组的克隆性和甲基化状态。

• DOI: 10.1002/ijc.2910480112
• 发表时间: 1991
• 期刊: International journal of cancer
• 影响因子: 6.4
• 作者: Lewin,N;Minarovits,J;Weber,G;Ehlin-Henriksson,B;Wen,T;Mellstedt,H;Klein,G;Klein,E
• 通讯作者: Klein,E

Evidence of tinidazole interference in the oropharyngeal inflammatory process during infectious mononucleosis.

替硝唑干扰传染性单核细胞增多症期间口咽炎症过程的证据。

• DOI: 10.3109/00365548609021654
• 发表时间: 1986
• 期刊: Scandinavian journal of infectious diseases
• 作者: Marklund,G;Lundberg,C;Nord,CE;Ernberg,I
• 通讯作者: Ernberg,I