Electrochemical sensor for monitoring levels of oxygen and nitrogen reactive species to benefit ageing research

用于监测氧和氮活性物质水平的电化学传感器有利于老化研究

• 批准号: BB/K013807/1
• 负责人: Bhavik Patel
• 金额: $ 12.03万
• 依托单位: University of Brighton
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2013
• 资助国家: 英国
• 起止时间: 2013 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FK013807%2F1
• 关键词: Electrochemical; sensor; monitoring; levels; oxygen

This proposal is focused on the development of a novel microelectrode that can be tailored for any specific chemical post biological and chemical modification. These microelectrodes will be made using carbon nanotube composite material, which allows the means to have a varied electrode area. These sensors can be made using two different strategies, where they can be held in either glass capillaries or plastic pipettes, allowing for sensors to be rigid or flexible. This is a rapid improvement compared to conventionally made carbon based electrodes. These sensors will be modified for the detection of various reactive oxygen and nitrogen species (ROS/RNS). These chemicals are of specific interest as they play key biological roles. They have been implicated in the ageing process and are the basis of many theories associated with the ageing process. At present, only total ROS/RNS have been detected with current technology, as the fluorescent dyes that are used offer poor specificity. This device will be made to benefit researchers working on ageing and is anticipated to increase the impact of these studies. Measurements will be made using microelectrode arrays, which will be fabricated using boron-doped diamond. These microelectrode arrays will be modified for detection of ROS/RNS. Our microelectrodes are advantageous as they allow simultaneous measurements of ROS/RNS over various locations.The ROS/RNS microelectrode and MEAs will be tested on two applications associated with ageing. The first will be to characterise the changes in ROS/RNS levels over various regions of old and young isolated neurones. The second application will be to look at ROS/RNS changes in cell lines that enter senescence. Measurements will be carried out in comparison with fluorescent dyes.Finally to allow access to this new sensor technology for bioscience researchers, we will host a workshop that will allow scientist to learn about how the sensor functions and how it can be utilised for monitoring ROS/RNS levels from biological samples. Overall this new sensing strategy will provide impact to studies associated with ageing, but with the main electrode having the ability to be modified for any specific analyte, the long-term benefits can be for various sectors and fields of importance. It is envisioned that this microelectrode can be tailored for detection of key analytes for major priority areas such as food security, understanding animal physiology and healthy ageing.

该提案的重点是开发一种新型微电极，该微电极可以针对任何特定的化学后生物和化学修饰进行定制。这些微电极将使用碳纳米管复合材料制成，这使得该装置具有不同的电极面积。这些传感器可以使用两种不同的策略来制造，它们可以容纳在玻璃毛细管或塑料移液管中，允许传感器是刚性或柔性的。与常规制造的碳基电极相比，这是快速的改进。这些传感器将被修改为检测各种活性氧和氮物质（ROS/RNS）。这些化学物质特别令人感兴趣，因为它们发挥着关键的生物作用。它们与衰老过程有关，是许多与衰老过程有关的理论的基础。目前，只有总ROS/RNS已被检测到与当前的技术，因为所使用的荧光染料提供不良的特异性。该设备将使从事老龄化研究的研究人员受益，预计将增加这些研究的影响。测量将使用微电极阵列，这将是使用硼掺杂金刚石制造。这些微电极阵列将被修改用于检测ROS/RNS。我们的微电极具有优势，因为它们可以同时测量不同位置的ROS/RNS。ROS/RNS微电极和MEA将在两个与老化相关的应用中进行测试。第一个将是在不同区域的老年和年轻的分离神经元的ROS/RNS水平的变化。第二个应用是观察进入衰老的细胞系中ROS/RNS的变化。最后，为了让生物科学研究人员能够接触到这种新的传感器技术，我们将举办一个研讨会，让科学家们了解传感器的功能以及如何利用它来监测生物样品中的ROS/RNS水平。总的来说，这种新的传感策略将对与老化相关的研究产生影响，但由于主电极能够针对任何特定分析物进行修改，因此长期受益可以用于各个重要部门和领域。据设想，这种微电极可以定制用于检测主要优先领域的关键分析物，如食品安全，了解动物生理学和健康老龄化。

项目成果

Chemically modified multiwall carbon nanotube composite electrodes: An assessment of fabrication strategies

• DOI: 10.1016/j.electacta.2014.11.129
• 发表时间: 2015-01-10
• 期刊: ELECTROCHIMICA ACTA
• 影响因子: 6.6
• 作者: Fagan-Murphy, Aidan;Allen, Marcus C.;Patel, Bhavik Anil
• 通讯作者: Patel, Bhavik Anil

Glucocorticoids induce production of reactive oxygen species/reactive nitrogen species and DNA damage through an iNOS mediated pathway in breast cancer.

• DOI: 10.1186/s13058-017-0823-8
• 发表时间: 2017-03-24
• 期刊: Breast cancer research : BCR
• 作者: Flaherty RL;Owen M;Fagan-Murphy A;Intabli H;Healy D;Patel A;Allen MC;Patel BA;Flint MS
• 通讯作者: Flint MS

Synthesis and applications of copillar[5]arene dithiols

• DOI: 10.1080/10610278.2015.1111375
• 发表时间: 2016-06-02
• 期刊: SUPRAMOLECULAR CHEMISTRY
• 影响因子: 3.3
• 作者: Kothur, Raghuram Reddy;Fucassi, Flavia;Cragg, Peter J.
• 通讯作者: Cragg, Peter J.