CHEMICAL CHARACTERISATION OF SYNAPTOGENESIS

突触发生的化学特征

• 批准号: EP/C532058/1
• 负责人: Bhavik Patel
• 金额: $ 17.78万
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Fellowship
• 财政年份: 2006
• 资助国家: 英国
• 起止时间: 2006 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FC532058%2F1
• 关键词: CHEMICAL; CHARACTERISATION; SYNAPTOGENESIS

Understanding the process of communication between neurones in the brain is important problem in both basic physiology and the understanding of disease. The role that neurotransmitters play during the formation of synapses is currently unknown. I intend with the use of a new type of diamond electrode (with Prof G. Swain, Michigan State University) and novel signal processing techniques (with Prof. M. Wightman, University of North Carolina) to understand the roles played by multiple co-released neurotransmitters during neuronal regeneration and synaptogenesis in culture (with Prof. N. Syed, University of Calgary). pH changes will also be measured as an indicator of vesicular release from neurones and will be used to validate the response of the diamond electrodeLong-term recordings (days) and intracellular from regenerating neurones in the intact CNS will be attempted for the first time, with Dr M. Yeoman (University of Brighton) and at Imperial CollegeThe transfer of technology between the institutions involved will help advance the means of analytical measurement in this important area and have broader technological application and consolidate my skills at the interface of analytical science, neurophysiology and engineering. The total cost of the project is 43,000.

了解大脑中神经元之间的通信过程是基础生理学和疾病认识的重要问题。神经递质在突触形成过程中的作用目前尚不清楚。我打算使用一种新型的金刚石电极（与G. Swain，密歇根州立大学）和新的信号处理技术（与M。Wightman教授，北卡罗来纳州大学），以了解多种共同释放的神经递质在培养的神经元再生和突触发生过程中所起的作用（与N。Syed，University of Calgary）. pH值的变化也将被测量作为神经元囊泡释放的指标，并将用于验证金刚石电极的反应。Yeoman（布莱顿大学）和帝国学院所涉及的机构之间的技术转让将有助于推进这一重要领域的分析测量手段，并具有更广泛的技术应用，巩固我在分析科学，神经生理学和工程学界面上的技能。该项目的总成本为43，000美元。

项目成果

High performance liquid chromatography method for the detection of released purinergic and biogenic amine signaling molecules from in vitro ileum tissue.

高效液相色谱法检测体​​外回肠组织释放的嘌呤能和生物胺信号分子。

• DOI: 10.1002/jssc.200900853
• 发表时间: 2010
• 期刊: Journal of separation science
• 影响因子: 3.1
• 作者: Lwin A

A study of the in-column detection performance for chromatography separation

• DOI: 10.1007/s10404-015-1561-9
• 发表时间: 2015-03
• 期刊: Microfluidics and Nanofluidics
• 影响因子: 2.8
• 作者: P. Leow;P. Chee;B. Patel;D. O’Hare

Electrochemical fecal pellet sensor for simultaneous real-time ex vivo detection of colonic serotonin signalling and motility.

• DOI: 10.1038/srep23442
• 发表时间: 2016-03-22
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Morris R;Fagan-Murphy A;MacEachern SJ;Covill D;Patel BA
• 通讯作者: Patel BA