ANIHWA call 1: MADISUP. Marek's Disease Virus induced immunosuppression: From diagnosis to vaccination

ANIHWA 电话 1：MADISUP。

• 批准号: BB/L014262/1
• 负责人: Venugopal Nair
• 金额: $ 40.19万
• 依托单位: The Pirbright Institute
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2014
• 资助国家: 英国
• 起止时间: 2014 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FL014262%2F1
• 关键词: ANIHWA; call; MADISUP.; Marek; Disease

Marek's disease virus (MDV) is a highly oncogenic alphaherpesvirus that causes high economic losses in poultry production. Beside acute death or extensive tumor formation, MDV induces immunosuppression (IS) thus increasing the susceptibility of chickens to other pathogens. Besides being an issue of animal health and welfare, it increases the need for pharmacological treatment and also for the frequency of vaccination against multitude of pathogens, as MDV infection was shown to strongly reduce vaccine responses. Furthermore, MDV vaccines themselves induce mild IS, as such causing a dilemma between the absolute necessity of a MDV vaccination and the reduced response to other vaccines.Though the phenomenon of IS has been recognised for decades, very little is known about the mechanisms behind MDV-induced IS to date. Our goal is to apply the unique expertise and the techniques developed over the last years by the consortium on MDV and chicken immunology to identify the basic mechanisms associated with IS, and to develop novel MDV vaccines with significantly reduced immunosuppressive properties.

马立克氏病病毒(MDV)是一种高度致癌的甲型疱疹病毒，在家禽生产中造成很高的经济损失。除了急性死亡或广泛的肿瘤形成外，MDV还可诱导免疫抑制，从而增加鸡对其他病原体的易感性。除了是动物健康和福利的问题外，它还增加了药物治疗的需要，也增加了对多种病原体的疫苗接种频率，因为MDV感染被证明大大降低了疫苗的反应。此外，MDV疫苗本身会引起轻微的IS，因此造成了在绝对必要的MDV疫苗接种和对其他疫苗反应减弱之间的两难境地。尽管IS现象已经被认识到几十年了，但迄今为止对MDV诱导IS背后的机制知之甚少。我们的目标是应用MDV和鸡免疫学联盟在过去几年中开发的独特专业知识和技术来确定与IS相关的基本机制，并开发显著降低免疫抑制特性的新型MDV疫苗。

项目成果

A simple and rapid approach to develop recombinant avian herpesvirus vectored vaccines using CRISPR/Cas9 system.

一种简单而快速的方法，是使用CRISPR/CAS9系统开发重组禽疱疹病毒矢量的。

• DOI: 10.1016/j.vaccine.2017.12.025
• 发表时间: 2018-01-29
• 期刊: Vaccine
• 影响因子: 5.5
• 作者: Tang N;Zhang Y;Pedrera M;Chang P;Baigent S;Moffat K;Shen Z;Nair V;Yao Y
• 通讯作者: Yao Y

Marek's disease virus oncoprotein Meq physically interacts with the chicken infectious anemia virus-encoded apoptotic protein apoptin.

• DOI: 10.18632/oncotarget.25628
• 发表时间: 2018-06-22
• 期刊: Oncotarget
• 作者: Brown AC;Reddy VRAP;Lee J;Nair V
• 通讯作者: Nair V

Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing.

使用 CRISPR/Cas9 基因编辑生成重组禽疱疹病毒载体。

• DOI: 10.3791/58193
• 发表时间: 2019
• 期刊: JoVE
• 作者: Tang N