The role of the Marek's disease virus (MDV) Meq oncoprotein in the pathogenesis of Marek's disease and the development of MDV vaccine strains
The role of the Marek's disease virus (MDV) Meq oncoprotein in the pathogenesis of Marek's disease and the development of MDV vaccine strains
批准号:
BB/G001642/1
负责人:
Venugopal Nair
金额:
$52.53万
依托单位:
依托单位国家:
英国
项目类别:
Research Grant
财政年份:
2009
资助国家:
英国
项目状态:
已结题
起止时间:
2009 至 --
中文摘要
马立克氏病(MD)是鸡的一种常见疾病,涉及瘫痪,并且通常由于高度恶性T淋巴瘤(白色血细胞的癌症)的生长而死亡。不寻常的癌症,MD是由一种可传播的媒介,马立克氏病病毒(MDV)。MDV具有很强的传染性,是全球家禽业的主要威胁,但可以通过接种疫苗加以控制。每年有超过50亿剂疫苗用于控制这种疾病。然而,病毒不断进化,毒力越来越强,因此需要新的疫苗。对该病毒获得毒力和产生肿瘤的机制仍然知之甚少。同样,决定有效疫苗株的因素仍然不清楚。在BBSRC的财政支持下,我们一直在研究一种名为Meq的蛋白质,它是由MDV在癌细胞中产生的。我们最近的研究结果已经明确表明,这种蛋白质对于MDV诱导肿瘤是绝对必要的(因此它被称为癌蛋白)。此外,它已经有可能确定特定的蛋白质:蛋白质相互作用涉及Meq是必要的,其功能作为一个癌蛋白。我们已经在Meq编码基因中进行了各种确定的突变,这些突变产生了减毒(非致病性)MDV,我们表明这些MDV可以用作疫苗。这是一个建议,以建立这项工作,以确定确切的贡献,Meq使马立克氏病的发展,在其各种病理表现/包括恶性和麻痹形式/并试图更好地了解什么是一个有效的疫苗株的MDV。
英文摘要
Marek's disease (MD) is a common disease of chickens involving paralysis and commonly death from the growth of highly malignant T lymphomas (cancers of white blood cells). Unusual for a cancer, MD is caused by a transmissible agent, Marek's disease virus (MDV). MDV is very contagious and is a major threat to the poultry industry worldwide, but it can be controlled by vaccination. More than 5 billion vaccine doses a year are used in an attempt to control the disease. However, there is a continuous evolution of the virus towards greater virulence and consequently new vaccines are required. The mechanisms by which the virus gains virulence and produces tumours remain very poorly understood. Similarly, the factors that determine an effective vaccine strain remain unclear. With financial support from the BBSRC, we have been studying a protein called Meq that is made by MDV in the cancer cells. Our recent results have demonstrated unequivocally that this protein is absolutely essential for the induction of tumours by MDV (it is therefore known as an oncoprotein). Moreover it has been possible to identify specific protein:protein interactions involving Meq that are necessary for its function as an oncoprotein. We have made various defined mutations in the Meq-encoding gene that produce attenuated (non-pathogenic) MDV that we showed can be used as vaccines. This is a proposal to build on this work in order to determine the precise contribution that Meq makes to the development of Marek's disease in its various pathological manifestations / including malignant and paralytic forms / and to try and understand better what constitutes an effective vaccine strain of MDV.
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Rate of natural transmission of Marek's disease virus (MDV) to susceptible chickens in the post-challenge period: effect of vaccination with HVT and association with MDV genome count in air.
攻击后马立克氏病病毒 (MDV) 向易感鸡的自然传播率:HVT 疫苗接种的影响以及与空气中 MDV 基因组计数的关联。
DOI:
--
发表时间:
2010
期刊:
Zootecnica International
影响因子:
--
作者:
[Crabb JR]
通讯作者:
Crabb JR
DOI:
10.1016/j.rvsc.2010.08.002
发表时间:
2011-08-01
期刊:
RESEARCH IN VETERINARY SCIENCE
影响因子:
2.4
作者:
[Baigent, S. J., Smith, L. P., Nair, V. K.]
通讯作者:
Nair, V. K.
Epigenetic regulation of the long repeats of Marek's Disease Virus (MDV) associated with latent and lytic expression profiles
马立克氏病病毒(MDV)长重复的表观遗传调控与潜伏和裂解表达谱相关
DOI:
--
发表时间:
2010
期刊:
5th International Workshop on the Molecular Pathogenesis of Marek's Disease Virus, University of Georgia, Athens, USA, 17-20 October 2010
影响因子:
--
作者:
[Brown A]
通讯作者:
Brown A
Induction of lymphomas by inoculation of Marek's disease virus-derived lymphoblastoid cell lines: prevention by CVI988 vaccination.
通过接种马立克氏病病毒衍生的淋巴母细胞系诱导淋巴瘤:通过 CVI988 疫苗接种进行预防。
DOI:
10.1080/03079457.2012.740159
发表时间:
2012
期刊:
journal of the W.V.P.A
影响因子:
--
作者:
[Mwangi WN]
通讯作者:
Mwangi WN
DOI:
10.1128/jvi.02828-14
发表时间:
2015-03
期刊:
Journal of virology
影响因子:
5.4
作者:
[Smith J, Sadeyen JR, Butter C, Kaiser P, Burt DW]
通讯作者:
Burt DW
US-UK Collab: The consequences of transmissible vaccines on disease ecology and pathogen evolution: Marek's disease virus as a case study
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批准号:BB/V017748/1
-
项目类别:Research Grant
-
资助金额:$81.81万
-
财政年份:2021
-
负责人:Venugopal Nair
-
依托单位:
Other Countries Partnering Award, South Korea: Genome editing and transgenic technologies for virus-host interaction studies in birds
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批准号:BB/M027481/1
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项目类别:Research Grant
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资助金额:$4.55万
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财政年份:2015
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负责人:Venugopal Nair
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依托单位:
ANIHWA call 1: MADISUP. Marek's Disease Virus induced immunosuppression: From diagnosis to vaccination
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依托单位:
European Partnering Award on Avian Disease Research (EPAADR)
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资助金额:$2.58万
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负责人:Venugopal Nair
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依托单位:
International workshop on "Recent Advances in Viral diseases of animals-implications on One Health"
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批准号:BB/K021206/1
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项目类别:Research Grant
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资助金额:$1.26万
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-
负责人:Venugopal Nair
-
依托单位:
Vaccines as drivers of disease emergence: transmission ecology and virulence evolution in Marek's disease
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批准号:BB/K011057/1
-
项目类别:Research Grant
-
资助金额:$126.4万
-
财政年份:2013
-
负责人:Venugopal Nair
-
依托单位:
Defining the cistrome and quantitative transcriptome of virus-transformed cells using massively parallel sequencing
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批准号:BB/I014284/1
-
项目类别:Research Grant
-
资助金额:$56.39万
-
财政年份:2012
-
负责人:Venugopal Nair
-
依托单位:
China-Vietnam-United Kingdom Partnership in combating viral diseases of poultry
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批准号:BB/J020257/1
-
项目类别:Research Grant
-
资助金额:$3.1万
-
财政年份:2012
-
负责人:Venugopal Nair
-
依托单位:
Marek's disease virus spread: In and out of chickens
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批准号:BB/I017577/1
-
项目类别:Research Grant
-
资助金额:$44.4万
-
财政年份:2011
-
负责人:Venugopal Nair
-
依托单位:
Translational control by non-coding RNA in Marek's disease herpesvirus: implications in oncogenesis and exploitation in bioengineering
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批准号:BB/H010696/1
-
项目类别:Research Grant
-
资助金额:$56.49万
-
财政年份:2010
-
负责人:Venugopal Nair
-
依托单位:
Studies leading to sustainable strategies for the control of Marek's disease: Is vaccination responsible for virulence evolution in Marek's disease?
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批准号:BB/E003540/2
-
项目类别:Research Grant
-
资助金额:$20.14万
-
财政年份:2009
-
负责人:Venugopal Nair
-
依托单位:
UK-India partnership on Marek's disease and RNA interference to control avian viral disease
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批准号:BB/G530325/1
-
项目类别:Research Grant
-
资助金额:$3.3万
-
财政年份:2009
-
负责人:Venugopal Nair
-
依托单位:
UK-US Collaboration on Exploiting the Cancer Epigenome in Animals
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批准号:BB/G530476/1
-
项目类别:Research Grant
-
资助金额:$6.54万
-
财政年份:2009
-
负责人:Venugopal Nair
-
依托单位:
Functional analysis of novel microRNAs encoded by avian herpesviruses
-
批准号:BB/G009163/1
-
项目类别:Research Grant
-
资助金额:$51.6万
-
财政年份:2009
-
负责人:Venugopal Nair
-
依托单位:
Combating highly pathogenic avian influenza: Novel vaccination strategies using recombinant live avian viral vaccine vectors. THIS GRANT IS A SUPPLEM
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批准号:BB/H531219/1
-
项目类别:Research Grant
-
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财政年份:2009
-
负责人:Venugopal Nair
-
依托单位:
Combating highly pathogenic avian influenza: Novel vaccination strategies using recombinant live avian viral vaccine vectors
-
批准号:BB/E01111X/1
-
项目类别:Research Grant
-
资助金额:$83.41万
-
财政年份:2008
-
负责人:Venugopal Nair
-
依托单位:
Differential co-expression in DNA microarray data and it's application to animal health
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批准号:BB/F013965/1
-
项目类别:Research Grant
-
资助金额:$47.51万
-
财政年份:2008
-
负责人:Venugopal Nair
-
依托单位:
Studies leading to sustainable strategies for the control of Marek's disease: Is vaccination responsible for virulence evolution in Marek's disease?
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批准号:BB/E003230/1
-
项目类别:Research Grant
-
资助金额:$57.3万
-
财政年份:2007
-
负责人:Venugopal Nair
-
依托单位:
Investigation into the role of host-pathogen molecular interactions in animal diseases by microscopy and live cell imaging
-
批准号:BB/E012795/1
-
项目类别:Research Grant
-
资助金额:$24.89万
-
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-
负责人:Venugopal Nair
-
依托单位:
Molecular events and mechanisms of Marek's disease pathogensis
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-
项目类别:Research Grant
-
资助金额:$21.99万
-
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-
负责人:Venugopal Nair
-
依托单位:
海外基金