DIAGNOSTIC RADIOLOGY IMAGING AND METABOLISM OF TUMOR

拟议的动物研究的总体目标是提供新的 与体内31P相关的信息-核磁共振(核磁共振) ~(11)C或~(18)F-2-脱氧葡萄糖(2DG)和~(11)C-蛋氨酸正电子的光谱 发射断层扫描在肿瘤代谢评价中的应用 在肿瘤生长的不同阶段，无论是未治疗的还是治疗的 (化疗)动物。更具体的目标包括对肿瘤进行高量化 用31P-核磁共振波谱测定能量磷酸盐代谢物和pH值(均在 体内和体外)，葡萄糖摄取和氨基酸摄取使用PET 生化扫描、肿瘤蛋白质合成和三磷酸腺苷水平 使用计算机轴位断层扫描(CT)的技术和肿瘤形态， 核磁共振质子成像和组织学。上述参数将在以下时间进行比较 两种动物肿瘤模型(兔VX-2后肢肌肉瘤和成人 犬脑瘤)在肿瘤生长及随访的不同阶段 无论是静脉化疗还是动脉化疗。研究结果 应提供与肿瘤生物学和 活体成像和光谱分析的潜在临床价值 监测肿瘤自然发展过程中代谢变化的技术 生长和后续治疗。结果的应用可以提供 监测癌症治疗和判断预后的新方法。

The overall goal of the proposed animal research is to provide new information relating in vivo 31P-Nuclear Magnetic Resonance (NMR) Spectroscopy to 11C or 18F-2-deoxyglucose (2DG) and 11C-Methionine Positron Emission Tomography (PET) Scanning in the evaluation of tumor metabolism during various stages of tumor growth in both untreated and treated (chemotherapy) animals. More specific aims include quantitating tumor high energy phosphate metabolites and pH using 31P-NMR Spectroscopy (both in vivo and in vitro), glucose uptake and amino acid uptake using PET Scanning, tumor protein synthesis and ATP levels using biochemical techniques and tumor morphology using computerized axial tomography (CT), NMR proton imaging and histology. The above parameters will be compared in two animal tumor models (Rabbit VX-2 hind limb muscle tumor and adult canine brain tumor) during various stages of tumor growth and following either intravenous or intraarterial chemotherapy. Results of the studies should provide important information relating to tumor biology and the potential clinical value of using in vivo imaging and spectroscopy techniques to monitor metabolic changes during the natural history of tumor growth and following therapy. The application of the results could provide a new approach to monitoring cancer therapy and determining prognosis.