13TSB_AgriFood: Optimising the delivery of superior genetics through advanced genomic selection of embryos

13TSB_AgriFood：通过先进的胚胎基因组选择优化卓越遗传学的传递

• 批准号: BB/L017393/1
• 负责人: Darren Griffin
• 金额: $ 26.7万
• 依托单位: University of Kent
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2013
• 资助国家: 英国
• 起止时间: 2013 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FL017393%2F1
• 关键词: 13TSB_AgriFood; Optimising; delivery; superior; genetics

This proposal is a natural but significant extension of two existing TSB funded projects involving the two collaboratingacademic institutions (i.e. TS/101069 ['Applying advanced breeding technologies to amplify and distribute bovine geneticsto Increase production efficiency and sustainability', involving the University of Nottingham], and TS/J003182/1 [Pig IVF andgenetics: a route to global sustainability, involving the University of Kent]) to develop advanced breeding technologies incattle with commercial partners (i.e. Paragon/XLVets, Cogent Breeding Ltd and Illumina Inc) that will allow the marketing ofembryos that have undergone a complete genomic evaluation (including sexing) in order to estimate the breeding value ofprogeny for traits of commercial importance at the earliest possible stage of development.This project will apply advanced breeding technologies to produce (both in-vivo and in-vitro) pre-implantation bovineembryos from which biopsies will be taken to interrogate their genomic makeup using single nucleotide polymorphismarrays (SNP chips). It is currently possible to screen dairy and beef cattle genomically, but responses to selection areimpeded by waiting for the gestation of the calf on which genomic selection is performed. The screening of bovineembryos, however, would optimise the delivery and amplification of superior genetics by advancing the time of selectionand reducing "wastage" of unwanted calves of inappropriate sex. This project will, therefore, combine advanced embryobreeding technologies with state-of-the-art genomic screening (so called pre-implantation genetic diagnosis), andkaryomapping (combining parental DNA information with the offspring's genomic information to provide more geneticdetail). It will develop strategies for: (i) optimal bovine embryo culture using sexed semen, (ii) embryo biopsy in order toextract a small number of blastomeres for genomic analysis, (iii) karyomapping SNP genotype data from MultipleDisplacement Amplification products and (iv) ehnancing pregnancy establishment of frozen-thawed embryos. Proof ofprinciple that the new technologies can be used to deliver superior genetics more efficiently to the breeding herd will beconfirmed by conducting genomic analyses of calves.Importantly, this project is geared towards the development and use of a unique and highly marketable product (i.e.genetically evaluated sexed embryos) that will deliver superior genetics to the global market at low cost, and in a highwelfare and bio-secure manner. This will have huge economic benefits for the UK based commercial companies concerned, and for both the dairy and beef sectors of the UK cattle industry; and ensure that the UK remains at the cutting edge of technical innovation retaining its competitive advantage over larger breeding companies currently situated inEurope, North and South America.

这项建议是TSB资助的两个现有项目的自然但重要的扩展，涉及两个合作的学术机构（即TS/101069“应用先进育种技术扩增和分配牛遗传学以提高生产效率和可持续性”，涉及诺丁汉大学）和TS/J 003182/1 [猪体外受精和遗传学：一条通往全球可持续发展的道路，涉及肯特大学），与商业伙伴一起开发先进的育种技术（即Paragon/XLVets，Cogent Breeding Ltd和Illumina Inc）将允许销售经过完整基因组评估的胚胎（包括性别鉴定），以便在尽可能早的发育阶段估计后代具有商业重要性的性状的育种价值。本项目将应用先进的育种技术，（包括体内和体外）植入牛胚胎，将从其取活组织检查以使用单核苷酸多态性芯片（SNP芯片）询问其基因组组成。目前，对奶牛和肉牛进行基因组筛选是可能的，但对选择的反应受到等待进行基因组选择的小牛妊娠的阻碍。然而，牛胚胎的筛选将通过提前选择时间和减少不需要的不适当性别小牛的“浪费”来优化上级遗传基因的传递和扩增。因此，该项目将把联合收割机先进的胚胎移植技术与最先进的基因组筛选（所谓的植入前遗传学诊断）和染色体作图（将亲本DNA信息与后代的基因组信息结合起来，以提供更多的遗传细节）结合起来。它将发展以下策略：（i）使用性别鉴定的精液进行最佳牛胚胎培养，（ii）胚胎活检以提取少量卵裂球用于基因组分析，（iii）从多重置换扩增产物中绘制SNP基因型数据，（iv）提高冻融胚胎的妊娠建立。通过对小牛进行基因组分析，将证实新技术可用于更有效地向种猪群提供上级遗传的原理证明。重要的是，该项目旨在开发和使用一种独特的、高度适销的产品（即经基因评估的性别胚胎），以低成本、高福利和生物安全的方式向全球市场提供上级遗传。这将为英国相关商业公司以及英国养牛业的乳制品和牛肉部门带来巨大的经济利益;并确保英国保持技术创新的前沿，保持其相对于目前位于欧洲、北美和南美的大型养殖公司的竞争优势。

项目成果

Preimplantation Genetic Testing for Aneuploidy Improves Live Birth Rates with In Vitro Produced Bovine Embryos: A Blind Retrospective Study.

• DOI: 10.3390/cells10092284
• 发表时间: 2021-09-02
• 期刊: Cells
• 影响因子: 6
• 作者: Silvestri G;Canedo-Ribeiro C;Serrano-Albal M;Labrecque R;Blondin P;Larmer SG;Marras G;Tutt DAR;Handyside AH;Farré M;Sinclair KD;Griffin DK
• 通讯作者: Griffin DK

Interaction of polyunsaturated fatty acids and melatonin during bovine in vitro maturation on cumulus-oocyte metabolism and embryo development. 1

牛体外成熟过程中多不饱和脂肪酸和褪黑激素对卵丘-卵母细胞代谢和胚胎发育的相互作用。

• 发表时间: 2022
• 作者: Tutt DAR

Analysis of the presence of mosaicism in bovine embryos through Preimplantation genetic testing (PGT-A) algorithms.

通过植入前基因检测 (PGT-A) 算法分析牛胚胎中嵌合体的存在。

• 发表时间: 2022
• 作者: Canedo-Ribeiro C

Preimplantation Genetic Testing for Aneuploidy (PGT-A) Reveals High Levels of Chromosomal Errors in In Vivo-Derived Pig Embryos, with an Increased Incidence When Produced In Vitro.

• DOI: 10.3390/cells12050790
• 发表时间: 2023-03-02
• 期刊: Cells
• 影响因子: 6

The effect of melatonin on in vitro maturation of bovine cumulus-oocyte complexes in the presence or absence of complex proteins.

在存在或不存在复合蛋白的情况下，褪黑激素对牛卵丘-卵母细胞复合物体外成熟的影响。

• 发表时间: 2022
• 作者: Tutt DAR