Commercialisation of a Super-Resolution multiphoton microscope

超分辨率多光子显微镜的商业化

• 批准号: BB/L024284/1
• 负责人: Nicholas Hartell
• 金额: $ 18.1万
• 依托单位: University of Leicester
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2014
• 资助国家: 英国
• 起止时间: 2014 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FL024284%2F1
• 关键词: Commercialisation; Super; Resolution; multiphoton; microscope

We have developed a new microscope that produces images with a resolution that is better than previously thought theoretically possible. The microscope uses an infrared laser for deep tissue imaging and combines structured illumination with software based localisation methods to resolve points of interest with nanometer resolution. We commissioned a market report and patent landscape survey to help develop a commercial strategy and confirm that we have freedom to operate. In response to comments from industry experts and end users, we will improve the depth of penetration to allow super-resolved imaging at depths of up to 0.5 mm. We will also develop a method for simultaneous measurement from two fluorescent markers and demonstrate the utility of these improvements using in vivo and in vitro and use the data to generate high impact papers. We will also protect the intellectual property underpinning our invention and speak with industry experts with a view to licencing our technology.

我们已经开发出一种新的显微镜，它产生的图像的分辨率比以前认为的理论上可能的要好。显微镜使用红外激光进行深层组织成像，并将结构化照明与基于软件的定位方法相结合，以纳米分辨率解析感兴趣的点。我们委托进行了市场报告和专利格局调查，以帮助制定商业战略并确认我们拥有运营自由。为了回应行业专家和最终用户的意见，我们将改进穿透深度，以实现深度达0.5 mm的超分辨成像。我们还将开发一种方法，用于同时测量两种荧光标记物，并使用体内和体外证明这些改进的实用性，并使用数据生成高影响力的论文。我们还将保护支持我们发明的知识产权，并与行业专家交谈，以获得我们技术的许可。

项目成果

Post-processing strategies in image scanning microscopy.

• DOI: 10.1016/j.ymeth.2015.05.002
• 发表时间: 2015-10
• 期刊: Methods
• 影响因子: 4.8
• 作者: J. McGregor;C. Mitchell;N. Hartell

Corrigendum: Imaging Calcium in Hippocampal Presynaptic Terminals With a Ratiometric Calcium Sensor in a Novel Transgenic Mouse.

勘误表：使用新型转基因小鼠中的比例钙传感器对海马突触前末梢中的钙进行成像。

• DOI: 10.3389/fncel.2019.00194
• 发表时间: 2019
• 期刊: Frontiers in cellular neuroscience
• 影响因子: 5.3
• 作者: Al-Osta I