MECHANISM OF REACTIONS OF BLEOMYCIN-IRON AND RELATED IRO

博莱霉素铁及相关 IRO 的反应机制

• 批准号: 3184034
• 负责人: TEDDY G. TRAYLOR
• 金额: $ 8.23万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-04-01 至 1989-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3184034
• 关键词: DNA; antineoplastics; bleomycin; carbon monoxide; chemical kinetics; chemical reaction; cyanides; heme; iron; nuclear magnetic resonance spectroscopy; oxidation; photochemistry; respiratory oxygen; ultraviolet spectrometry

It is proposed to study the kinetics and mechanisms of reaction of bleomycin-iron(II) and several synthetic analogues with carbin monixide, isonitriles and dioxygen in order to clarify the mechanisms of its activation by dioxygen and the effect of DNA binding on this activation. The reaction of BLM-Fe(II) with oxygen atom donors and subsequent substrate oxidation will be compared with similar processes using hemin catalysts. Oxidation of a DNA "monomer unit" will be carried out using BLM-Fe and hemin catalysts in order to determine the mechanism of this reaction. New analogues of BLM will be prepared and tested as DNA cleaving reagents.

提出了研究反应动力学和反应机理的方法。 博来霉素-铁（II）和几种具有一氧化碳的合成类似物， 异腈和分子氧，以澄清其机制， 分子氧激活和DNA结合对这种激活的影响。 BLM-Fe（II）与氧原子供体及后续底物的反应 氧化将与使用氯化血红素催化剂的类似工艺进行比较。 DNA“单体单元”的氧化将使用BLM-Fe进行， 氯化血红素催化剂，以确定该反应的机理。 新 将制备BLM的类似物并作为DNA切割试剂进行测试。