MECHANISM OF REACTIONS OF BLEOMYCIN-IRON AND RELATED IRO

博莱霉素铁及相关 IRO 的反应机制

• 批准号: 3184035
• 负责人: TEDDY G. TRAYLOR
• 金额: $ 7.46万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-04-01 至 1989-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3184035
• 关键词: DNA; antineoplastics; bleomycin; carbon monoxide; chemical kinetics; chemical reaction; cyanides; heme; iron; nuclear magnetic resonance spectroscopy; oxidation; photochemistry; respiratory oxygen; ultraviolet spectrometry

It is proposed to study the kinetics and mechanisms of reaction of bleomycin-iron(II) and several synthetic analogues with carbin monixide, isonitriles and dioxygen in order to clarify the mechanisms of its activation by dioxygen and the effect of DNA binding on this activation. The reaction of BLM-Fe(II) with oxygen atom donors and subsequent substrate oxidation will be compared with similar processes using hemin catalysts. Oxidation of a DNA "monomer unit" will be carried out using BLM-Fe and hemin catalysts in order to determine the mechanism of this reaction. New analogues of BLM will be prepared and tested as DNA cleaving reagents.

拟研究反应动力学和机理 博来霉素铁(II)和几种与一氧化碳合成的类似物， 异腈和分子氧，以阐明其作用机制 双氧的激活以及 DNA 结合对此激活的影响。 BLM-Fe(II) 与氧原子供体和后续底物的反应 氧化反应将与使用氯化血红素催化剂的类似过程进行比较。 DNA“单体单元”的氧化将使用 BLM-Fe 和 氯化血红素催化剂以确定该反应的机理。 新的 BLM 类似物将被制备并作为 DNA 切割试剂进行测试。