UPGRADE OF LASER FACILITY FOR THE STUDY OF FAST KINETICS

六个研究小组研究结构，动力学和功能， 生物聚合物及其与小分子-配体和药物的相互作用 - 正在申请激光设施的共用仪器， 快速动力学和时间分辨光谱研究。 该设施将 研究结果表明：（1）CO、O_2、NO结合到 各种天然血红蛋白，无论是在特定的人类 病理学和作为蛋白质功能的模型，2）机制 为了将这种小配体结合到模拟血红蛋白的模型化合物上， 细胞色素P450和其他蛋白质，以了解 蛋白质中活性位点周围的特定辅基，3） 核酸的结构及其与药物的相互作用以及 核酸运动的动力学，它可以调节活性， 受药物影响，4）抗精神病药物的分子形式，其 两亲分子结构提出了一个简单的解释， 与细胞膜结合，但其活性形式现在似乎是某种 不同，5）流体相中分子反应的基本特征， 对于任何系统来说，这一点还不太清楚，但现在可以 在真实的生物系统中进行真正的基础研究，以及6） 肽激素和合成类似物的结构和功能。 的 所要求的设备包括最近扩大和升级a 建立设施，以提供更宽的光谱范围，更好地 时间分辨率，以及所有用户的更多可访问性。 当这些 添加到现有组件，该设施将能够 测量紫外、可见和近红外时间分辨光谱， 利用吸收、发光和拉曼散射的反应动力学 从相对较慢的（大约百万分之一秒）时间 大聚合物和细胞单位的活性特征， 时间低于万亿分之一秒，在这个时间尺度上， 发生了基本的分子过程。

Six research groups studying the structure, dynamics, and function of biopolymers and their interactions with small molecules - ligands and drugs - are applying for shared instrumentation for a Laser Facility for the Study of Fast Kinetics and Time Resolved Spectroscopy. The Facility will be used to investigate: 1) The mechanism of binding CO, O2, and NO to a variety of natural hemoglobins, both in relation to particular human pathologies and as a model of protein function in general, 2) The mechanism for binding such small ligands to model compounds which mimic hemoglobin, cytochrome P450, and other proteins in order to understand the role of particular prosthetic groups around active sites in proteins, 3) The structure of nucleic acids and their interactions with drugs as well as the dynamics of nucleic acid motion, which may regulate activity and is affected by drugs, 4) The molecular form of antipsychotic drugs, whose amphipathic molecular structures had suggested a simple explanation for binding to membranes, but whose active form now seems to be something quite different, 5) The basic features of molecular reactions in fluid phases, which are not really known in any detail for any system but which now can be studied at a truly fundamental level in real biological systems, and 6) The structure and function of peptide hormones and synthetic analogs. The equipment requested includes components to expand and upgrade a recently established facility in order to provide broader spectral range, better time resolution, and more accessibility all the users. When these additions are added to existing components, the facility will be able to measure ultraviolet, visible and near infrared time-resolved spectra and kinetics of reactions using absorption, luminescence, and Raman scattering from the comparatively slow (approximately millionth of a second) times characteristic of the activity of large polymers and cellular units down to times below a trillionth of a second, the time scale on which the most clementary and fundamental molecular processes occur.