UPGRADE OF LASER FACILITY FOR THE STUDY OF FAST KINETICS

升级激光设备以研究快速动力学

• 批准号: 3519142
• 负责人: TEDDY G. TRAYLOR
• 金额: $ 21.38万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-12-01 至 1985-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3519142
• 关键词: chemical group; chemical structure; drug metabolism; fluorescent dye /probe; hemoprotein structure; laser spectrometry; lasers; peptide hormone; photomultiplier

Six research groups studying the structure, dynamics, and function of biopolymers and their interactions with small molecules - ligands and drugs - are applying for shared instrumentation for a Laser Facility for the Study of Fast Kinetics and Time Resolved Spectroscopy. The Facility will be used to investigate: 1) The mechanism of binding CO, O2, and NO to a variety of natural hemoglobins, both in relation to particular human pathologies and as a model of protein function in general, 2) The mechanism for binding such small ligands to model compounds which mimic hemoglobin, cytochrome P450, and other proteins in order to understand the role of particular prosthetic groups around active sites in proteins, 3) The structure of nucleic acids and their interactions with drugs as well as the dynamics of nucleic acid motion, which may regulate activity and is affected by drugs, 4) The molecular form of antipsychotic drugs, whose amphipathic molecular structures had suggested a simple explanation for binding to membranes, but whose active form now seems to be something quite different, 5) The basic features of molecular reactions in fluid phases, which are not really known in any detail for any system but which now can be studied at a truly fundamental level in real biological systems, and 6) The structure and function of peptide hormones and synthetic analogs. The equipment requested includes components to expand and upgrade a recently established facility in order to provide broader spectral range, better time resolution, and more accessibility all the users. When these additions are added to existing components, the facility will be able to measure ultraviolet, visible and near infrared time-resolved spectra and kinetics of reactions using absorption, luminescence, and Raman scattering from the comparatively slow (approximately millionth of a second) times characteristic of the activity of large polymers and cellular units down to times below a trillionth of a second, the time scale on which the most clementary and fundamental molecular processes occur.

六个研究小组研究了细胞的结构、动力学和功能 生物聚合物及其与小分子、配体和药物的相互作用 -正在为激光设备申请共享仪器，用于 快速动力学和时间分辨光谱研究。该设施将 用于研究：1)CO、O2和NO与 天然血红蛋白的种类，都与特定的人类有关 病理和作为蛋白质功能模型的一般，2)机制 为了将这样的小配体结合到模拟血红蛋白的模型化合物上， 为了了解细胞色素P450等蛋白质的作用 蛋白质中活性部位周围的特殊修复基团，3) 核酸的结构及其与药物的相互作用 核酸运动的动力学，它可以调节活动，并 受药物影响，4)抗精神病药物的分子形式，其 两亲性分子结构给出了一个简单的解释 结合到膜上，但其活性形式现在似乎是一种 不同，5)流体中分子反应的基本特征， 对于任何系统来说都不是真正知道的细节，但现在可以 在真实的生物系统中进行真正基础的研究，以及6) 多肽激素及其合成类似物的结构和功能。这个 所需设备包括扩展和升级最近的 建立设施，以提供更宽的光谱范围，更好 时间分辨率更高，更便于所有用户使用。当这些 添加到现有组件后，该设施将能够 测量紫外线、可见光和近红外的时间分辨光谱和 利用吸收、发光和拉曼散射的反应动力学 从相对较慢(大约百万分之一秒)的时间 大分子聚合物和细胞单元的活性特征 乘以万亿分之一秒以下的时间，在这个时间尺度上 基本的和基本的分子过程发生了。