FIBER VOLATILE FATTY ACIDS AND COLONIC CELL BIOLOGY

纤维挥发性脂肪酸和结肠细胞生物学

• 批准号: 3181156
• 负责人: SHARON E FLEMING
• 金额: $ 13.78万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-09-01 至 1990-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3181156
• 关键词: acid base balance; autoradiography; bicarbonates; biopsy; blood chemistry; cell differentiation; cell growth regulation; cellulose; dietary fiber; dietary lipid; fatty acids; feces analysis; fermentation; gastrointestinal motility /pressure; gastrointestinal nutrient absorption; grain; intestinal mucosa; microorganism metabolism; mucins; nutrition related neoplasm /cancer; nutrition related tag; pectins

Volatile fatty acids (VFA) are produced when dietary or endogenous constituents are fermented by microbes in the colon. Some dietary fibers stimulate VFA formation. VFA have been shown to influence differentiation and proliferation of normal and malignant colinic mucosal cells, and it has been postulated that VFA may also influence the pH of the colon, and colonic function. Each of these factors appears to influence the risk of colonic carcinogenesis. Since the appropriate specimens which are needed to study the interactions of dietary fiber, VFA and colonic cell biology cannot be acquired from healthy humans, we have developed the pig as an appropriate animal model. In this study, we postulate that VFA production in the gut can be altered by feeding different dietary fibers, and that the fibers may also influence absorption of VFA into the colonic mucosal cells and blood. We further postulate that the biology of the mucosal cells will be influenced by the quantity of VFA which are absorbed, and that the lumenal environment will also be affected. To test our hypothesis, pigs will be fed diets containing purified or semi-purified fibers which are selected to show a range in solubility, physical properties and fermentability. To determine the influence of these fibers on VFA production, absorption and excretion, VFA will be measured in cecal contents, portal blood and feces. To determine the influence on cell biology, cecal and colonic biopsy specimens will be examined for cell proliferation and differentiation. The relationships of dietary fiber, VFA production and absorption, colonic cell biology and the lumenal environment of the colon will be determined so that we can more accurately select fibers which are likely to offer protection against colonic disease. This study is designed to provide data which will identify the specific effects that fibers have on the colonic environment and on the colonic cell biology of a healthy animal. From these data we may understand why there are often conflicting reports on the effect of a fiber on tumorigenesis, and we can then go on to further examine those fibers which are most likely to protect the colon against cancer.

挥发性脂肪酸（VFA）是由膳食或内源性产生的

项目成果

The influence of dietary fiber on proliferation of intestinal mucosal cells in miniature swine may not be mediated primarily by fermentation.

膳食纤维对小型猪肠粘膜细胞增殖的影响可能不是主要通过发酵介导的。

• DOI: 10.1093/jn/122.4.906
• 发表时间: 1992
• 期刊: The Journal of nutrition
• 作者: Fleming,SE;Fitch,MD;DeVries,S
• 通讯作者: DeVries,S

Role of dietary factors in cell replication and colon cancer.

饮食因素在细胞复制和结肠癌中的作用。

• DOI: 10.1093/ajcn/48.3.775
• 发表时间: 1988
• 期刊: The American journal of clinical nutrition
• 作者: Jacobs,R

Effect of dietary fiber on colonic cell proliferation and its relationship to colon carcinogenesis.

膳食纤维对结肠细胞增殖的影响及其与结肠癌发生的关系。

• DOI: 10.1016/0091-7435(87)90073-9
• 发表时间: 1987
• 期刊: Preventive medicine
• 影响因子: 5.1
• 作者: Jacobs,LR

Dietary fat and fiber: relationship to caloric intake, body growth, and colon tumorigenesis.

膳食脂肪和纤维：与热量摄入、身体生长和结肠肿瘤发生的关系。

• DOI: 10.1093/ajcn/45.1.243
• 发表时间: 1987
• 期刊: The American journal of clinical nutrition
• 作者: Nauss,KM;Jacobs,LR;Newberne,PM
• 通讯作者: Newberne,PM