Expansion and Further Development of the PSIPRED Protein Structure and Function Bioinformatics Workbench

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With many genomes now completely sequenced, life scientists now face the challenge of characterizing the biological role of the encoded proteins as to advance our understanding of cell physiology. Most genes are designed to code for proteins which have useful functions in an organism. Proteins are essentially strings of simpler molecules, called amino acids and these strings can self-assemble into a complex 3-D structure as soon as the protein is formed by the protein-making machinery (ribosomes) in the cell. It is this unique structure which determines the precise chemical function of the protein (i.e. what is does in the cell and how it does it). By firing X-rays at crystallised proteins, scientists can determine their structure, but this process can take many months or even years. With hundreds of thousands of proteins for which the native structure is unknown, it is not surprising that scientists want to find a clever shortcut to working out the structure of proteins. We, like many other scientists have been trying to "crack the code" of protein structure i.e. working out the rules which govern how the protein finds its unique structure and then trying to program a computer with these rules to allow scientists to quickly "predict" what the structure of their protein of interest might be.The PSIPRED Workbench is a collection of Web servers maintained at UCL which does just this i.e. it allows biologists to predict the structure of their protein structure given just its amino acid sequence. Over the years it has helped many thousands of scientists with their work by providing these services and we now wish not only to upgrade and maintain these existing servers but also to implement new methods which allow the structures of even the most difficult proteins to be deduced by computer simulations.More recently, however, PSIPRED has been given a wider range of features to cover other important problems in biology. For example, using PSIPRED, a scientist can predict which proteins do not fold into stable shapes (called disordered proteins) or which chemical substances are likely to bind to a protein. Even where a protein does not appear to fold into a single stable structure, PSIPRED can still help scientists deduce what the function of his or her protein is likely to be. Generating such information on a large scale using computer algorithms can help expand our knowledge base of the biological role of proteins at a cellular level, and such understanding will be a key stepping stone in the development of techniques and pharmaceuticals to target diseased genes and their products as well as proteins from pathological organisms such as bacteria or viruses. In a similar way, knowledge on the function of certain bacterial genes can, for example, help develop new industrial processes by modifying the genes to make them produce novel chemical compounds, or even helping to detoxify industrial waste by producing friendly bacteria that can use the poisonous chemicals as food.