High Resolution Mass Spectrometer to support Proteomic Research across the Southern 4 Proteomic Consortium

高分辨率质谱仪支持 Southern 4 蛋白质组学联盟的蛋白质组学研究

• 批准号: BB/M012387/1
• 负责人: Christopher Proud
• 金额: $ 57.17万
• 依托单位: University of Southampton
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2015
• 资助国家: 英国
• 起止时间: 2015 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FM012387%2F1
• 关键词: Resolution; Mass; Spectrometer; support; Proteomic

New instrumentation for increased capability in Proteomic ResearchProteins are a major building block of cells and perform a huge range of biological functions. Proteomics is the systematic study of the expression, functions and interactions of proteins. The key techniques used in proteomics are mass spectrometry (MS) and liquid chromatography and are performed on instruments called Mass Spectrometers; and High Performance Liquid Chromatography (HPLC) systems. In combination, these two instruments are designed to separate and identify proteins, fragments of proteins (peptides) and modified proteins obtained from cells, tissues and biofluids. They allow us to compare the amounts of proteins between different conditions (e.g., comparison of protein levels between normal cells and cancer cells), and to identify proteins important within a specific biological process including normal ones and those important in human diseases. This application will support the purchase of an advanced Mass Spectrometer and HPLC system with an enhanced ability to measure the amounts of these proteins and peptides at very low levels and with high accuracy.The instrumentation has been requested by the Southern 4 Proteomic Consortium and will form part of a Proteomics hub in the South of England that will allow several universities to use this state-of-the-art instrumentation. This consortium also provides a forum to exchange proteomic expertise and knowledge; and to train researchers in the latest proteomic techniques. Housed within the Centre for Proteomic Research at the University of Southampton, the increased capacity and advanced capabilities of this instrumentation will permit a wide range of proteomic studies within areas of strategic importance, including those within many BBSRC priority areas including healthy aging across the lifecourse; bioenergy, generating new replacement fuels for a greener, sustainable future; food, nutrition and health; sustainability enhancing agricultural production; systems approaches to the biosciences; synthetic biology and data-driven biology.

提高蛋白质组研究能力的新仪器蛋白质是细胞的主要组成部分，具有广泛的生物学功能。蛋白质组学是对蛋白质的表达、功能和相互作用的系统研究。蛋白质组学中使用的关键技术是质谱仪(MS)和液相色谱(LC)，它们是在称为质谱仪的仪器上进行的；以及高效液相色谱(HPLC)系统。这两种仪器相结合，旨在分离和鉴定从细胞、组织和生物体液中获得的蛋白质、蛋白质片段(多肽)和修饰蛋白质。它们使我们能够比较不同条件下的蛋白质含量(例如，比较正常细胞和癌细胞之间的蛋白质水平)，并识别特定生物过程中的重要蛋白质，包括正常蛋白质和人类疾病中的重要蛋白质。这项应用将支持购买先进的质谱仪和高效液相系统，以增强在非常低的水平和高精度测量这些蛋白质和多肽的数量的能力。应南方4蛋白质联盟的要求，该仪器将成为英格兰南部蛋白质组学中心的一部分，该中心将允许几所大学使用这种最先进的仪器。该联盟还提供了一个交流蛋白质组学专业知识和培训研究人员最新蛋白质组学技术的论坛。该仪器设在南安普顿大学蛋白质组学研究中心内，能力的提高和先进能力的提高将有助于在具有战略重要性的领域进行广泛的蛋白质组学研究，包括在BBSRC许多优先领域进行的研究，包括整个生命周期的健康老龄化；生物能源，为更绿色、可持续的未来生产新的替代燃料；粮食、营养和健康；增强农业生产的可持续性；生物科学的系统方法；合成生物学和数据驱动生物学。

项目成果

Asthma similarities across ProAR (Brazil) and U-BIOPRED (Europe) adult cohorts of contrasting locations, ethnicity and socioeconomic status

• DOI: 10.1016/j.rmed.2019.105817
• 发表时间: 2020-01-01
• 期刊: RESPIRATORY MEDICINE
• 影响因子: 4.3
• 作者: Cruz, Alvaro A.;Riley, John H.;Chung, Kian Fan
• 通讯作者: Chung, Kian Fan

A computational framework for complex disease stratification from multiple large-scale datasets.

• DOI: 10.1186/s12918-018-0556-z
• 发表时间: 2018-05-29
• 期刊: BMC systems biology
• 作者: De Meulder B;Lefaudeux D;Bansal AT;Mazein A;Chaiboonchoe A;Ahmed H;Balaur I;Saqi M;Pellet J;Ballereau S;Lemonnier N;Sun K;Pandis I;Yang X;Batuwitage M;Kretsos K;van Eyll J;Bedding A;Davison T;Dodson P;Larminie C;Postle A;Corfield J;Djukanovic R;Chung KF;Adcock IM;Guo YK;Sterk PJ;Manta A;Rowe A;Baribaud F;Auffray C;U-BIOPRED Study Group and the eTRIKS Consortium
• 通讯作者: U-BIOPRED Study Group and the eTRIKS Consortium

Large-Scale Label-Free Quantitative Mapping of the Sputum Proteome

• DOI: 10.1021/acs.jproteome.8b00018
• 发表时间: 2018-06-01
• 期刊: JOURNAL OF PROTEOME RESEARCH
• 影响因子: 4.4
• 作者: Burg, Dominic;Schofield, James P. R.;Skipp, Paul J.
• 通讯作者: Skipp, Paul J.

The immunopeptidomes of two transmissible cancers and their host have a common, dominant peptide motif.

• DOI: 10.1111/imm.13307
• 发表时间: 2021-06
• 期刊: Immunology
• 影响因子: 6.4
• 作者: Gastaldello A;Ramarathinam SH;Bailey A;Owen R;Turner S;Kontouli N;Elliott T;Skipp P;Purcell AW;Siddle HV
• 通讯作者: Siddle HV

Proteomic analysis of haptenation by skin sensitisers: Diphencyprone and ethyl acrylate.

皮肤致敏剂：联苯环酮和丙烯酸乙酯的半抗原化的蛋白质组学分析。

• DOI: 10.1016/j.tiv.2019.104697
• 发表时间: 2020
• 期刊: an international journal published in association with BIBRA
• 作者: Parkinson E