Regulation of the male germline in cereal crops by somatic companion cells

体细胞伴随细胞对谷类作物雄性种系的调节

• 批准号: BB/N00194X/1
• 负责人: Jose Gutierrez-Marcos
• 金额: $ 57.7万
• 依托单位: University of Warwick
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2016
• 资助国家: 英国
• 起止时间: 2016 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FN00194X%2F1
• 关键词: Regulation; male; germline; cereal; crops

Like animals, most diploid plants reproduce sexually after going through genetic reduction (meiosis) to produce specialized haploid gametes (i.e. sperm cells and egg cells) within the male and female sex organs, respectively. However, the process by which plants establish haploid sexual lineages (or germlines) from diploid somatic tissues late in adult life is not yet understood, but is thought to involve large-scale developmental and whole-genome reprogramming. This proposal focuses on uncovering some of the mechanisms involved in this process. We have evidence that novel small-RNA binding Argonaute proteins (AGOs) specifically found in male reproductive organs of cereals and their associated class of small non-coding RNA molecules control the development of the male germline. Intriguingly, these AGOs are expressed in somatic cells surrounding the male germline and sperm cells, yet defects or mutations in these AGOs causes male sterility. In this proposal, we will use various approaches and experimental techniques (eg. developmental genetics, biochemistry, computational and molecular biology) to determine exactly how somatic companion cells regulate the fate of the male germline using two important model crop plants: maize and wheat. We anticipate that this work will not only advance our basic understanding of how plants set their male germline, but will also provide a novel strategy to engineer new breeding tools in agronomically important cereal crops.

像动物一样，大多数二倍体植物在经历遗传减数分裂后进行有性繁殖，分别在雄性和雌性性器官中产生专门的单倍体配子(即精子细胞和卵细胞)。然而，植物在成年后期从二倍体体细胞组织建立单倍体有性系(或生殖系)的过程尚不清楚，但被认为涉及大规模的发育和全基因组重新编程。这项提议的重点是揭示这一过程中涉及的一些机制。我们有证据表明，在谷物雄性生殖器官中特异发现的新的小RNA结合ArgAerte蛋白(AGOS)及其相关的一类非编码小RNA分子控制着雄性生殖系的发育。有趣的是，这些agos在男性生殖系和精子细胞周围的体细胞中表达，但这些agos的缺陷或突变会导致男性不育。在这个提案中，我们将使用各种方法和实验技术(例如，发展遗传学、生物化学、计算和分子生物学)，利用两种重要的模式作物：玉米和小麦，准确地确定体细胞伴生细胞如何控制雄性生殖系的命运。我们预计，这项工作不仅将增进我们对植物如何设置雄性生殖系的基本理解，还将为设计具有重要农学意义的谷类作物的新育种工具提供一种新的策略。

项目成果

A new role for histone demethylases in the maintenance of plant genome integrity.

• DOI: 10.7554/elife.58533
• 发表时间: 2020-10-27
• 期刊: eLife
• 影响因子: 7.7
• 作者: Antunez-Sanchez J;Naish M;Ramirez-Prado JS;Ohno S;Huang Y;Dawson A;Opassathian K;Manza-Mianza D;Ariel F;Raynaud C;Wibowo A;Daron J;Ueda M;Latrasse D;Slotkin RK;Weigel D;Benhamed M;Gutierrez-Marcos J
• 通讯作者: Gutierrez-Marcos J

Article

• DOI: 10.1111/j.1944-8287.2000.tb00151.x
• 发表时间: 2000-10
• 期刊: Economic Geography
• 影响因子: 7
• 作者: Arifin Musthafa

A Novel Signaling Pathway Required for Arabidopsis Endodermal Root Organization Shapes the Rhizosphere Microbiome.

• DOI: 10.1093/pcp/pcaa170
• 发表时间: 2021-05-11
• 期刊: Plant & cell physiology
• 影响因子: 4.9
• 作者: Durr J;Reyt G;Spaepen S;Hilton S;Meehan C;Qi W;Kamiya T;Flis P;Dickinson HG;Feher A;Shivshankar U;Pavagadhi S;Swarup S;Salt D;Bending GD;Gutierrez-Marcos J
• 通讯作者: Gutierrez-Marcos J

Specific chromatin changes mark lateral organ founder cells in the Arabidopsis inflorescence meristem

• DOI: 10.1093/jxb/erz181
• 发表时间: 2019-08-01
• 期刊: JOURNAL OF EXPERIMENTAL BOTANY
• 影响因子: 6.9
• 作者: Frerichs, Anneke;Engelhorn, Julia;Werr, Wolfgang
• 通讯作者: Werr, Wolfgang

A transposon surveillance mechanism that safeguards plant male fertility during stress

• DOI: 10.1038/s41477-020-00818-5
• 发表时间: 2021-01-04
• 期刊: NATURE PLANTS
• 影响因子: 18
• 作者: Lee, Yang-Seok;Maple, Robert;Gutierrez-Marcos, Jose
• 通讯作者: Gutierrez-Marcos, Jose