14 ERA-CAPS: INvestigating TRiticeae EPIgenomes for Domestication (INTREPID)

14 ERA-CAPS：研究小麦科 EPI 基因组用于驯化 (INTREPID)

• 批准号: BB/N005104/2
• 负责人: Anthony Hall
• 金额: $ 39.47万
• 依托单位: Earlham Institute
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2016
• 资助国家: 英国
• 起止时间: 2016 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FN005104%2F2
• 关键词: 14; ERA; CAPS; INvestigating; TRiticeae

Plant breeding uses DNA sequence variation to make new allelic combinations for crop improvement. Our creation of the first wheat gene sequence assemblies (Brenchley et al Nature 491, 705) has enabled new levels of high throughput precise genotyping for breeding this globally important crop. Nevertheless, there are other levels of heritable variation, such as epigenetic modifications, that are widely thought to play a key role in shaping genomes and creating new variation. We have recently developed highly efficient re-sequencing technologies for wheat that can measure DNA methylation in genes of multiple lines. This provides an outstanding opportunity to assess epigenetic variation in a major polyploid crop and understand how it may influence traits. The overall objective of this proposal is to use newly available wheat genome resources, together with our innovative application of exome capture and bisulphite sequencing, to measure epigenetic modifications in wheat genes, and relate these to gene expression and the acquisition of new phenotypes, and how they may contribute to genetic changes such as gene loss during polyploid formation.

植物育种利用DNA序列变异产生新的等位基因组合用于作物改良。我们创建了第一个小麦基因序列组装体（Brenchley等Nature 491，705），使得高通量精确基因分型的新水平成为可能，用于育种这种全球重要的作物。然而，还有其他水平的遗传变异，如表观遗传修饰，被广泛认为在塑造基因组和创造新的变异中发挥关键作用。我们最近开发了高效的小麦重测序技术，可以测量多个品系基因中的DNA甲基化。这为评估主要多倍体作物的表观遗传变异和了解它如何影响性状提供了一个绝佳的机会。该提案的总体目标是使用新的可用小麦基因组资源，以及我们对外显子组捕获和亚硫酸氢盐测序的创新应用，来测量小麦基因的表观遗传修饰，并将这些与基因表达和新表型的获得联系起来，以及它们如何可能有助于遗传变化，例如多倍体形成期间的基因丢失。

项目成果

Reduced chromatin accessibility underlies gene expression differences in homologous chromosome arms of hexaploid wheat and diploid Aegilops tauschii

• DOI: 10.1101/571133
• 发表时间: 2019-03
• 期刊: bioRxiv
• 作者: Fu-Hao Lu;N. McKenzie;Laura-Jayne Gardiner;M. Luo;Anthony Hall;M. Bevan

Mapping-by-sequencing in complex polyploid genomes using genic sequence capture: a case study to map yellow rust resistance in hexaploid wheat.

• DOI: 10.1111/tpj.13204
• 发表时间: 2016-08
• 期刊: The Plant journal : for cell and molecular biology
• 作者: Gardiner LJ;Bansept-Basler P;Olohan L;Joynson R;Brenchley R;Hall N;O'Sullivan DM;Hall A
• 通讯作者: Hall A