BIOCHEMISTRY OF SALIVARY LIPIDS

这个项目的长期目标是探索生物化学和 唾液和唾液结石中脂质的功能与健康和健康 疾病，并确定这些脂质与蛋白质和 糖蛋白成分。为拟议期间确定的具体目标 是：确定共价结合和相关的脂类在 唾液粘蛋白的浮力密度、粘度和疏水行为 从防龋者(CR)和易龋者(CS)； 评估共价结合和相关脂类对 唾液粘蛋白对蛋白质降解的敏感性；分离 并对CR和CS受试者的腮腺唾液中的脂蛋白进行表征； 测定共价结合的脂类的组成和含量 与腮腺唾液中富含脯氨酸的糖蛋白相关，并评估 与龋病的区别；探讨血脂与龋齿的关系 牙釉质膜中的蛋白质和糖蛋白；评价其作用 膜粘蛋白和脂蛋白成分在迟缓中的作用 致龋酸的扩散和脂类对这一功能的贡献 指膜；描述覆盖口腔的粘液层的脂类 上皮细胞，探索它们的起源及其与其他成分的关系， 并测定该粘液的流变性和渗透性 目的：探讨慢性阻塞性肺疾病患者口腔粘膜层脂质的变化。 口腔炎的受试者；为了研究底物特异性， 酶的亚细胞定位和动力学 大鼠唾液中糖蛋白与脂肪酸的酰化反应 腺体；进行酰基转移酶活性的测量 涎腺和全身疾病患者的唾液腺活检 疾病；测量负责硫酸盐化的酶的活性 慢性前列腺炎患者唾液腺分泌糖脂和结构糖脂的研究 唾液腺和全身性疾病；以及继续研究血脂和 它们与食管虫分泌物中的蛋白质和糖蛋白的关系 和小涎腺患者的涎腺和全身性 疾病。

The long term objective of this project is to explore the biochemistry and function of lipids in saliva and salivary concretions in health and disease, and to define the relationship of these lipids to the protein and glycoprotein components. The specific goals set for the proposed period are: to establish the role of covalently bound and associated lipids in the buoyant density, viscosity and hydrophobic behavior of salivary mucins from caries-resistant (CR) and caries-susceptible (CS) subjects; to evaluate the effect of covalently bound and associated lipids on the susceptibility of salivary mucins to proteolytic degradation; to isolate and characterize lipoproteins of parotid saliva from CR and CS subjects; to determine the composition and content of lipids covalently bound and associated with proline-rich glycoprotein of parotid saliva, and assess differences with caries; to investigate the relationship of lipids to proteins and glycoproteins in tooth enamel pellicle; to evaluate the role of pellicle mucin and lipoprotein components in the retardation of cariogenic acids diffusion and the contribution of lipids to this function of pellicle; to characterize lipids of the mucous layer covering human oral epithelium, explore their origin and relationship to other constituents, and determine rheological and permselective properties of this mucous layer; to investigate changes in lipids of the oral mucous layer in subjects with aphthous stomatitis; to study substrate specificity, subcellular location and kinetics of the enzyme responsible for the acylation of salivary glycoproteins with fatty acids in rat salivary glands; to conduct the measurements of the acyltransferase activity in salivary gland biopsies of patients with salivary gland and systemic disease; to measure the activities of enzymes responsible for sulfation of secretory and structural glycolipids in salivary glands of patients with salivary gland and systemic disease; and to pursue studies on lipids and their relation to proteins and glycoproteins in the secretions of the major and minor salivary glands of patients with salivary gland and systemic diseases.