BIOCHEMISTRY OF SALIVARY LIPIDS

唾液脂质的生物化学

• 批准号: 3219560
• 负责人: BRONISLAW LESZEK SLOMIANY
• 金额: $ 21.68万
• 依托单位: UNIV OF MED/DENT OF NJ-NJ MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-06-01 至 1992-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3219560
• 关键词: biochemistry; carbohydrates; dental caries; dental deposit; gingiva disorder; glucose; glycolipids; human subject; laboratory rat; lipid biosynthesis; lipids; molecular biology; oral bacteria; parotid gland; periodontium disorder; phospholipids; saliva; sublingual gland; submandibular gland; sulfation

The long term objective of this project is to explore the biochemistry and function of lipids in saliva and salivary concretions in health and disease, and to define the relationship of these lipids to the protein and glycoprotein components. The specific goals set for the proposed period are: to establish the role of covalently bound and associated lipids in the buoyant density, viscosity and hydrophobic behavior of salivary mucins from caries-resistant (CR) and caries-susceptible (CS) subjects; to evaluate the effect of covalently bound and associated lipids on the susceptibility of salivary mucins to proteolytic degradation; to isolate and characterize lipoproteins of parotid saliva from CR and CS subjects; to determine the composition and content of lipids covalently bound and associated with proline-rich glycoprotein of parotid saliva, and assess differences with caries; to investigate the relationship of lipids to proteins and glycoproteins in tooth enamel pellicle; to evaluate the role of pellicle mucin and lipoprotein components in the retardation of cariogenic acids diffusion and the contribution of lipids to this function of pellicle; to characterize lipids of the mucous layer covering human oral epithelium, explore their origin and relationship to other constituents, and determine rheological and permselective properties of this mucous layer; to investigate changes in lipids of the oral mucous layer in subjects with aphthous stomatitis; to study substrate specificity, subcellular location and kinetics of the enzyme responsible for the acylation of salivary glycoproteins with fatty acids in rat salivary glands; to conduct the measurements of the acyltransferase activity in salivary gland biopsies of patients with salivary gland and systemic disease; to measure the activities of enzymes responsible for sulfation of secretory and structural glycolipids in salivary glands of patients with salivary gland and systemic disease; and to pursue studies on lipids and their relation to proteins and glycoproteins in the secretions of the major and minor salivary glands of patients with salivary gland and systemic diseases.

这个项目的长期目标是探索生物化学和