BIOCHEMISTRY OF SALIVARY LIPIDS

唾液脂质的生物化学

• 批准号: 3219563
• 负责人: BRONISLAW LESZEK SLOMIANY
• 金额: $ 14.97万
• 依托单位: UNIV OF MED/DENT OF NJ-NJ MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-06-01 至 1992-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3219563
• 关键词: biochemistry; carbohydrates; dental caries; dental deposit; gingiva disorder; glucose; glycolipids; human subject; laboratory rat; lipid biosynthesis; lipids; molecular biology; oral bacteria; parotid gland; periodontium disorder; phospholipids; saliva; sublingual gland; submandibular gland; sulfation

The long term objective of this project is to explore the biochemistry and function of lipids in saliva and salivary concretions in health and disease, and to define the relationship of these lipids to the protein and glycoprotein components. The specific goals set for the proposed period are: to establish the role of covalently bound and associated lipids in the buoyant density, viscosity and hydrophobic behavior of salivary mucins from caries-resistant (CR) and caries-susceptible (CS) subjects; to evaluate the effect of covalently bound and associated lipids on the susceptibility of salivary mucins to proteolytic degradation; to isolate and characterize lipoproteins of parotid saliva from CR and CS subjects; to determine the composition and content of lipids covalently bound and associated with proline-rich glycoprotein of parotid saliva, and assess differences with caries; to investigate the relationship of lipids to proteins and glycoproteins in tooth enamel pellicle; to evaluate the role of pellicle mucin and lipoprotein components in the retardation of cariogenic acids diffusion and the contribution of lipids to this function of pellicle; to characterize lipids of the mucous layer covering human oral epithelium, explore their origin and relationship to other constituents, and determine rheological and permselective properties of this mucous layer; to investigate changes in lipids of the oral mucous layer in subjects with aphthous stomatitis; to study substrate specificity, subcellular location and kinetics of the enzyme responsible for the acylation of salivary glycoproteins with fatty acids in rat salivary glands; to conduct the measurements of the acyltransferase activity in salivary gland biopsies of patients with salivary gland and systemic disease; to measure the activities of enzymes responsible for sulfation of secretory and structural glycolipids in salivary glands of patients with salivary gland and systemic disease; and to pursue studies on lipids and their relation to proteins and glycoproteins in the secretions of the major and minor salivary glands of patients with salivary gland and systemic diseases.

这个项目的长期目标是探索生物化学和 唾液和唾液凝固物中脂质的功能， 疾病，并确定这些脂质与蛋白质的关系， 糖蛋白组分。 为拟议期间确定的具体目标 是：建立共价结合和相关的脂质的作用， 唾液粘蛋白浮力密度、粘度和疏水性 来自抗龋（CR）和龋敏感（CS）受试者; 评价共价结合和缔合的脂质对 唾液粘蛋白对蛋白水解降解敏感性;分离 和表征来自CR和CS受试者的腮腺唾液的脂蛋白; 确定共价结合的脂质的组成和含量， 与腮腺唾液中富含脯氨酸的糖蛋白相关，并评估 与龋齿的差异;调查脂质与 蛋白质和糖蛋白在牙釉质膜中的作用 表膜粘蛋白和脂蛋白成分在延缓 致龋酸扩散和脂质对该功能的贡献 表膜;表征覆盖人类口腔粘膜层的脂质 上皮，探索它们的起源和与其他成分的关系， 并确定这种粘液的流变学和选择性渗透特性 层;调查在口腔粘膜层脂质的变化， 患有口疮性口炎的受试者;为了研究底物特异性， 亚细胞定位和酶的动力学负责 大鼠唾液糖蛋白与脂肪酸的酰化反应 腺;进行酰基转移酶活性的测量， 唾液腺和全身性疾病患者的唾液腺活检 疾病;测量负责硫酸化的酶的活性， 唾液腺分泌和结构糖脂 唾液腺和全身性疾病;以及进行脂质和 它们与主要哺乳动物分泌物中的蛋白质和糖蛋白的关系 唾液腺和全身性疾病患者的小唾液腺 疾病