BIOCHEMISTRY OF SALIVARY LIPIDS

唾液脂质的生物化学

• 批准号: 3219561
• 负责人: BRONISLAW LESZEK SLOMIANY
• 金额: $ 21.39万
• 依托单位: UNIV OF MED/DENT OF NJ-NJ MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-06-01 至 1992-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3219561
• 关键词: acylation; aminoacyltransferase; aphthous stomatitis; biochemistry; carbohydrates; dental caries; dental deposit; enzyme mechanism; gingiva disorder; glucose; glycolipids; glycoproteins; human subject; laboratory rat; lipid biosynthesis; lipids; molecular biology; mucins; nonblood lipoprotein; oral bacteria; parotid gland; pellicle; periodontium disorder; phospholipids; proline; proteolysis; saliva; salivary disorder; secretion; sulfation; tooth enamel

The long term objective of this project is to explore the biochemistry and function of lipids in saliva and salivary concretions in health and disease, and to define the relationship of these lipids to the protein and glycoprotein components. The specific goals set for the proposed period are: to establish the role of covalently bound and associated lipids in the buoyant density, viscosity and hydrophobic behavior of salivary mucins from caries-resistant (CR) and caries-susceptible (CS) subjects; to evaluate the effect of covalently bound and associated lipids on the susceptibility of salivary mucins to proteolytic degradation; to isolate and characterize lipoproteins of parotid saliva from CR and CS subjects; to determine the composition and content of lipids covalently bound and associated with proline-rich glycoprotein of parotid saliva, and assess differences with caries; to investigate the relationship of lipids to proteins and glycoproteins in tooth enamel pellicle; to evaluate the role of pellicle mucin and lipoprotein components in the retardation of cariogenic acids diffusion and the contribution of lipids to this function of pellicle; to characterize lipids of the mucous layer covering human oral epithelium, explore their origin and relationship to other constituents, and determine rheological and permselective properties of this mucous layer; to investigate changes in lipids of the oral mucous layer in subjects with aphthous stomatitis; to study substrate specificity, subcellular location and kinetics of the enzyme responsible for the acylation of salivary glycoproteins with fatty acids in rat salivary glands; to conduct the measurements of the acyltransferase activity in salivary gland biopsies of patients with salivary gland and systemic disease; to measure the activities of enzymes responsible for sulfation of secretory and structural glycolipids in salivary glands of patients with salivary gland and systemic disease; and to pursue studies on lipids and their relation to proteins and glycoproteins in the secretions of the major and minor salivary glands of patients with salivary gland and systemic diseases.

该项目的长期目标是探索生物化学和 唾液中的脂质和唾液分泌物对健康和健康的作用 疾病，并定义这些脂质与蛋白质和 糖蛋白成分。 拟议期间设定的具体目标 是：确定共价结合和相关脂质在 唾液粘蛋白​​的浮力密度、粘度和疏水行为 来自抗龋齿 (CR) 和易龋齿 (CS) 受试者；到 评估共价结合和相关脂质对 唾液粘蛋白​​对蛋白水解降解的敏感性；隔离 并表征 CR 和 CS 受试者腮腺唾液的脂蛋白；到 确定共价结合的脂质的组成和含量 与腮腺唾液富含脯氨酸的糖蛋白相关，并评估 与龋齿的区别；研究脂质与 牙釉质表层中的蛋白质和糖蛋白；评估角色 皮膜粘蛋白和脂蛋白成分在延缓 致龋酸扩散以及脂质对此功能的贡献 薄膜；表征覆盖人类口腔的粘膜层的脂质 上皮细胞，探索它们的起源以及与其他成分的关系， 并确定该粘液的流变学和渗透选择性特性 层;研究口腔粘膜层脂质的变化 患有口疮性口炎的受试者；研究底物特异性， 负责该酶的亚细胞定位和动力学 大鼠唾液中脂肪酸对唾液糖蛋白的酰化 腺体；进行酰基转移酶活性的测量 唾液腺和全身患者的唾液腺活检 疾病;测量负责硫酸化的酶的活性 患者唾液腺中的分泌糖脂和结构糖脂 唾液腺和全身疾病；并进行脂质和 它们与主要分泌物中蛋白质和糖蛋白的关系 唾液腺和全身患者的小唾液腺 疾病。