PRODUCTION AND ABSORPTION OF INTESTINAL GAS

本提案的总体目标是调查 胃肠道通过测量气体的产生和吸收 在肚子里。 将对这些一般领域进行调查。 1)结肠细菌在酒精性器官损伤中的作用 伤害将被调查。 为此，将收集粪便样本 以及粪便细菌产生的挥发性代谢物 将通过气相色谱法测量与乙醇孵育期间的浓度。 的 这些代谢物的产生将与以下物质的存在相关： 各种与酒精有关的疾病。 如果观察到相关性， 这一发现可能有助于解释 对乙醇范围的敏感性，并有助于直接预防措施。 将在体内评估肠道中未搅拌水层的影响 使用一种新技术，该技术采用一氧化碳（CO）作为探针。 而 这一层已经在体外进行了广泛的研究，缺乏技术， 在体内测量这一层对其生理特性的了解有限， 意义 所描述的研究将利用CO吸收 测量以定量未搅拌层的厚度并评估 它对各种营养素吸收率的影响。 一个可能 这项研究的结果可能是信息，使之有可能 通过操纵未搅拌层来增强或减少吸收。 结肠细菌产生和消耗H2将是 研究了 本研究的新颖之处在于H2的产生和H2 消费将同时测量，而所有以前的研究， 粪便细菌的H2“生产”已经测量了净H2生产。 的 这项研究的结果应该阐明一些基本因素， 影响碳水化合物吸收不良的H2呼气试验，确定 引起和直接治疗胀气，并加强我们的理解， 粪便细菌和结肠之间的复杂关系 环境

The general goal of this proposal is to investigate the function of the gastrointestinal tract by measuring the production and absorption of gases in the gut. These general areas will be investigated. 1) The possible role of the colonic bacteria in alcohol-induced organ injury will be investigated. To this end, fecal samples will be collected from alcoholics and the volatile metabolites produced by the fecal bacteria during incubation with ethanol will be measured by gas chromatography. The production of these metabolites will be correlated with the presence of various forms of alcohol related disease. If a correlation is observed, this finding could help to explain the individual differences in susceptibility to the ranges of ethanol and help direct preventive measures. The influence of unstirred water layers in the gut will be assessed in vivo using a new technique which employs carbon monoxide (CO) as a probe. While this layer has been extensively studied in vitro, the lack of techniques to measure this layer in vivo has limited understanding of its physiological significance. The studies described will utilize CO absorption measurements to quantitate the thickness of the unstirred layer and assess its influence on the absorption rate of a variety of nutrients. A possible outgrowth of this study may be information which makes it possible to enhance or decrease absorption by manipulation of the unstirred layer. The production and consumption of H2 by colonic bacteria will be investigated. The novel aspect of this study is that H2 production and H2 consumption will be measured simultaneously whereas all previous studies of H2 "production" by fecal bacteria have measured net H2 production. The results of this study should elucidate some of the fundamental factors influencing the H2 breath test for carbohydrate malabsorption, identify the cause and direct therapy for flatulence, and enhance our understanding of the complex relationship between the fecal bacteria and the colonic environment.