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PRODUCTION AND ABSORPTION OF INTESTINAL GAS

肠道气体的产生和吸收

基本信息

批准号：
3150858
负责人：
MICHAEL D LEVITT
金额：
$ 6.83万
依托单位：
UNIVERSITY OF MINNESOTA
依托单位国家：
美国
项目类别：
财政年份：
1979
资助国家：
美国
起止时间：
1979-01-01 至 1986-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3150858
关键词：
carbon monoxide colon enteric bacteria gas chromatography gastrointestinal absorption /transport gastrointestinal circulation gastrointestinal circulation disorder gastrointestinal disorder diagnosis gastrointestinal gas inborn carbohydrate metabolism disorder malabsorption malnutrition methane nutrition related tag radiotracer

项目摘要

The general goal of this proposal is to investigate the function of the gastrointestinal tract by measuring the production and absorption of gases in the gut. These general areas will be investigated. 1) The possible role of the colonic bacteria in alcohol-induced organ injury will be investigated. To this end, fecal samples will be collected from alcoholics and the volatile metabolites produced by the fecal bacteria during incubation with ethanol will be measured by gas chromatography. The production of these metabolites will be correlated with the presence of various forms of alcohol related disease. If a correlation is observed, this finding could help to explain the individual differences in susceptibility to the ranges of ethanol and help direct preventive measures. The influence of unstirred water layers in the gut will be assessed in vivo using a new technique which employs carbon monoxide (CO) as a probe. While this layer has been extensively studied in vitro, the lack of techniques to measure this layer in vivo has limited understanding of its physiological significance. The studies described will utilize CO absorption measurements to quantitate the thickness of the unstirred layer and assess its influence on the absorption rate of a variety of nutrients. A possible outgrowth of this study may be information which makes it possible to enhance or decrease absorption by manipulation of the unstirred layer. The production and consumption of H2 by colonic bacteria will be investigated. The novel aspect of this study is that H2 production and H2 consumption will be measured simultaneously whereas all previous studies of H2 "production" by fecal bacteria have measured net H2 production. The results of this study should elucidate some of the fundamental factors influencing the H2 breath test for carbohydrate malabsorption, identify the cause and direct therapy for flatulence, and enhance our understanding of the complex relationship between the fecal bacteria and the colonic environment.

这项提案的总体目标是调查通过测量胃肠道气体的产生和吸收在肠子里。将对这些一般领域进行调查。 1)结肠细菌在酒精诱导器官中的可能作用将对受伤情况进行调查。为此，将收集粪便样本来自酒精和粪便细菌产生的挥发性代谢物在与乙醇孵育过程中，将用气相色谱进行测定。这个这些代谢物的产生将与存在的各种形式的酒精相关疾病。如果观察到相关性，这一发现可能有助于解释老年人的个体差异对乙醇范围的敏感性，并有助于指导预防措施。肠道中未搅拌的水层的影响将在体内进行评估。使用了一种使用一氧化碳(CO)作为探针的新技术。而当这一层已经在体外进行了广泛的研究，但缺乏技术来在体内测量这一层对其生理了解有限意义。所描述的研究将利用CO吸收测量未搅拌层的厚度并进行评估它对多种营养素的吸收速度有影响。一个可能的这项研究的结果可能是使我们有可能通过操纵未搅动的层来增强或减少吸收。结肠菌产生和消耗氢气将是调查过了。这项研究的新颖之处在于，氢气的产生和氢气消费将同时进行衡量，而之前所有关于粪便细菌的氢气“生产”已经测量到净氢气产量。这个这项研究的结果应该能阐明一些基本因素影响H2呼气试验的碳水化合物吸收不良，确定对胃肠胀气的病因和直接治疗，并增进我们对粪便细菌与结肠的复杂关系环境。