Genomics-led improvement of biotic and abiotic stress tolerance in mustard rape for economic and environmental sustainability

基因组学主导提高芥菜生物和非生物胁迫耐受性，实现经济和环境可持续发展

• 批准号: BB/R019819/1
• 负责人: Ian Bancroft
• 金额: $ 224.77万
• 依托单位: University of York
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FR019819%2F1
• 关键词: Genomics; led; improvement; biotic; abiotic

The proposed research is a Newton-Bhabha development programme. The overall aim is to transfer and optimize UK expertise in genomics, which is the scientific approach involving analysis in parallel of the complete set of genes of an organism, for improvement of both economic and environmental sustainability of mustard rape (Brassica juncea) in India. The crop characteristics (traits) that are the focus of the research were defined by the Indian partners in the proposal as the most important challenges faced by the crop in India. There are tolerances to the range of environmental challenges (stresses): diseases and infestations from fungi (causing white rust, stem rot, black spot), viruses (Turnip mosaic virus), pests (aphids and butterflies) and root parasites (broomrape) and conditions of high temperatures, drought and salinity. Of particular importance is that multiple stresses are often encountered simultaneously and interactions and trade-offs between tolerance mechanisms can be expected, necessitating an integrated approach across this broad range of challenges. Included in a broad correlation analysis between the traits will be an assessment of associations with variation in classes of chemicals produced by the plants that are recognised as playing roles in tolerance to environmental stresses. To enable this programme to be undertaken thoroughly and successfully, we have assembled a consortium comprising 39 co-applicant scientists representing 17 institutions. The approach is to establish a toolkit of technologies to help understand the basis of naturally-occurring tolerances and to enable future work to enhance them. These include the establishment of a platform enabling the association of trait variation in panels of genetically diverse mustard rape varieties with variation of both gene sequences and gene activity (expression) to enable the development of molecular markers to accelerate breeding and identify candidate causative genes for further investigation. Non-GM approaches for improvement beyond the range of existing natural variation will be established for mustard rape, including modernised resources for the traditional approaches of radiation breeding and wide crossing with related species, and the emerging technology of genome editing.Underpinning the programme is the experience gained in developing the Brassica juncea genomic platform currently used by the University of York and University of Delhi South Campus as part of their current Crop Genomics and Technologies (CGAT) project "Broadening the genetic diversity underpinning seed quality and yield related traits in mustard rape and oilseed rape" will be updated to incorporate emerging genome sequences from B. juncea and its progenitor species. The platform will be used to support the trait-focussed activities of the consortium, modelled on the University of York-led "BBSRC Renewable Industrial Products from rapeseed (RIPR) programme". A particular feature will be the highly integrated nature of the research with expertise contributed by world-leaders in the respective components being shared. UK expertise will be transferred to partners in India for application in mustard rape. The benefits of scientific understanding and ability to improve traits in mustard rape accrue primarily to the Indian members of the consortium. However, they will also be of use for improving the corresponding traits in oilseed rape for cultivation in the UK.

拟议的研究是一个牛顿-巴巴发展方案。总体目标是转让和优化英国在基因组学方面的专业知识，这是一种涉及平行分析生物体全套基因的科学方法，以改善印度芥菜油菜（Brassica juncea）的经济和环境可持续性。作为研究重点的作物特性（性状）被印度合作伙伴在提案中定义为印度作物面临的最重要挑战。对一系列环境挑战（压力）有耐受力：真菌（引起白色锈病、茎腐病、黑斑病）、病毒（芜菁花叶病毒）、害虫（蚜虫和蝴蝶）和根部寄生虫（肉苁蓉）造成的疾病和感染，以及高温、干旱和盐碱条件。特别重要的是，往往同时遇到多种压力，可以预期容忍机制之间会相互作用和权衡，因此需要对这一广泛的挑战采取综合办法。在性状之间的广泛相关性分析中，将评估与植物产生的化学物质类别变化的关联，这些化学物质被认为在环境胁迫耐受性中发挥作用。为了使这项计划能够彻底和成功地进行，我们已经组建了一个由代表17个机构的39名共同申请科学家组成的财团。该方法是建立一个技术工具包，以帮助了解自然发生的公差的基础，并使未来的工作，以提高他们。这些措施包括建立一个平台，使小组的遗传多样性芥菜油菜品种的性状变异与基因序列和基因活性（表达）的变化，使分子标记的发展，以加速育种和确定候选致病基因的进一步调查。将为芥菜油菜建立超越现有自然变异范围的非转基因改良方法，包括用于辐射育种和与相关物种远缘杂交的传统方法的现代化资源，以及新兴的基因组编辑技术。该计划的基础是约克大学和德里大学南校区目前使用的开发芥菜基因组平台的经验作为他们当前作物基因组学和技术（CGAT）项目“拓宽芥菜油菜和油菜种子质量和产量相关性状的遗传多样性基础”的一部分，将更新以纳入来自B的新兴基因组序列。juncea及其祖先物种。该平台将用于支持该联盟以性状为重点的活动，以约克大学领导的“BBSRC油菜籽可再生工业产品（RIPR）计划”为模型。一个特别的特点是研究的高度综合性，世界领导人在各自的组成部分中贡献了专门知识。英国的专业知识将转让给印度的合作伙伴，用于芥菜油菜。科学理解和改善芥菜油菜性状的能力的好处主要归于该财团的印度成员。然而，它们也将用于改善在英国栽培的油菜的相应性状。

项目成果

Water-related innovations in land plants evolved by different patterns of gene cooption and novelty.

• DOI: 10.1111/nph.17981
• 发表时间: 2022-07
• 期刊: NEW PHYTOLOGIST
• 影响因子: 9.4
• 作者: Bowles, Alexander M. C.;Paps, Jordi;Bechtold, Ulrike
• 通讯作者: Bechtold, Ulrike

Characterization and Mapping of retr04, retr05 and retr06 Broad-Spectrum Resistances to Turnip Mosaic Virus in Brassica juncea, and the Development of Robust Methods for Utilizing Recalcitrant Genotyping Data.

• DOI: 10.3389/fpls.2021.787354
• 发表时间: 2021
• 期刊: Frontiers in plant science
• 影响因子: 5.6
• 作者: Bramham LE;Wang T;Higgins EE;Parkin IAP;Barker GC;Walsh JA
• 通讯作者: Walsh JA

Identification of Chromosomes and Chromosome Rearrangements in Crop Brassicas and Raphanus sativus: A Cytogenetic Toolkit Using Synthesized Massive Oligonucleotide Libraries.

• DOI: 10.3389/fpls.2020.598039
• 发表时间: 2020
• 期刊: Frontiers in plant science
• 影响因子: 5.6
• 作者: Agrawal N;Gupta M;Banga SS;Heslop-Harrison JP
• 通讯作者: Heslop-Harrison JP

Azole fungicide sensitivity and molecular mechanisms of reduced sensitivity in Irish Pyrenopeziza brassicae populations.

爱尔兰 Pyrenopeziza 芸苔种群中唑类杀菌剂敏感性和敏感性降低的分子机制。

• DOI: 10.1002/ps.7219
• 发表时间: 2022
• 期刊: Pest management science
• 影响因子: 4.1
• 作者: Bucur DE

Modelling of gene loss propensity in the pangenomes of three Brassica species suggests different mechanisms between polyploids and diploids.

• DOI: 10.1111/pbi.13674
• 发表时间: 2021-12
• 期刊: Plant biotechnology journal
• 影响因子: 13.8
• 作者: Bayer PE;Scheben A;Golicz AA;Yuan Y;Faure S;Lee H;Chawla HS;Anderson R;Bancroft I;Raman H;Lim YP;Robbens S;Jiang L;Liu S;Barker MS;Schranz ME;Wang X;King GJ;Pires JC;Chalhoub B;Snowdon RJ;Batley J;Edwards D
• 通讯作者: Edwards D