18-BTT: Induction of double haploids in wheat using CENH3 mutants and genome editing

传统的小麦育种需要六到七个世代的自花授粉，才能提供高度纯合和稳定的品种。另一种方法是产生加倍单倍体(DH)，这可以缩短世代时间，将真正的繁育系传递给单一的世代。目前小麦的水解酶技术(基于玉米授粉)是费力的，需要高水平的专业知识和设备齐全的实验室。这些都是昂贵的资源，即使在发达国家也严重限制了卫生署的使用。在模式物种拟南芥中，一种名为CENH3的基因的氨基酸变化导致了单倍体诱导(HI)系。我们提出了一种策略，将该基因的突变体和基因编辑版本相结合，以改变小麦中的CENH3，并培育单倍体诱导系。我们的最终目标是通过开发高效的单倍体诱导系来加速小麦育种，这些单倍体诱导系将产生加倍的单倍体作为种子。

Traditional wheat breeding requires six to seven generations of self-pollination to deliver highly homozygous and stable cultivars. An alternative approach is the production of doubled haploids (DH) which reduces the generation time to deliver true-breeding lines to a single generation. Current DH technologies in wheat (based on maize pollination) are laborious and require a high level of expertise and well-equipped laboratories. These are expensive resources and severely limit DH use even in developed countries. Amino acid changes in a gene called CENH3 result in haploid inducing (HI) lines in the model species Arabidopsis. We propose a strategy to combine mutants and gene-edited versions of this gene to alter CENH3 in wheat and develop haploid inducer lines. Our final goal is to accelerate wheat breeding by developing efficient haploid inducer lines that will produce doubled haploids (DH) as seeds