UVR8 photoreceptor action and regulation

UVR8光感受器的作用和调节

• 批准号: BB/T003251/1
• 负责人: Gareth Islwyn Jenkins
• 金额: $ 58.89万
• 依托单位: University of Glasgow
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FT003251%2F1
• 关键词: UVR8; photoreceptor; action; regulation

Ultraviolet-B (UV-B) radiation is a minor but very energetic component of sunlight. Exposure to UV-B wavelengths (280-315 nm) has numerous effects on plants, including changes in metabolism and development that impact on biochemical composition, plant architecture and defence. The effects of UV-B are due to its ability to regulate the expression of numerous plant genes, including those involved in metabolic pathways, UV-protection, morphogenesis and defence against pests and pathogens. It is therefore important to understand how UV-B is perceived by plants and how it initiates responses. A key component involved in these processes is a protein called UVR8, which acts as a photoreceptor to detect UV-B light. UVR8 physically interacts with other proteins, COP1 and a number of transcription factors, to initiate responses to UV-B in plants. Further proteins, termed RUP proteins repress UVR8 activity. However, we have little understanding of the molecular mechanisms that determine how UVR8 interacts differentially with these proteins to control its activity, although we know that it involves a particular region of the UVR8 protein. Hence the aim of this project is to understand how UVR8 interacts with key regulatory proteins. In addition to establishing the molecular basis of UVR8 action, the information gained opens up the possibility of identifying variants of UVR8 and its interacting proteins that would allow manipulation of UVR8 activity in crop plants to enhance aspects of quality and productivity.

紫外线-B（UV-B）辐射是太阳光中一种微小但能量很大的成分。暴露于紫外线B波长（280-315纳米）对植物有许多影响，包括影响生物化学组成、植物结构和防御的新陈代谢和发育的变化。紫外线B的影响是由于它能够调节许多植物基因的表达，包括那些参与代谢途径、紫外线防护、形态发生和抵御害虫和病原体的基因。因此，了解植物如何感知UV-B以及它如何引发反应非常重要。参与这些过程的一个关键成分是一种称为UVR 8的蛋白质，它作为光受体来检测UV-B光。UVR 8与其他蛋白质、COP 1和许多转录因子相互作用，启动植物对UV-B的反应。另外的蛋白质，称为RUP蛋白质，抑制UVR 8活性。然而，我们对决定UVR 8如何与这些蛋白质差异地相互作用以控制其活性的分子机制知之甚少，尽管我们知道它涉及UVR 8蛋白的特定区域。因此，该项目的目的是了解UVR 8如何与关键调控蛋白相互作用。除了建立UVR 8作用的分子基础外，所获得的信息还开辟了鉴定UVR 8及其相互作用蛋白的变体的可能性，这些变体将允许操纵作物植物中的UVR 8活性以提高质量和生产力。