OXIDATIVE PHOSPHORYLATION IN HEART MITOCHONDRIA

心脏线粒体的氧化磷酸化

• 批准号: 3268528
• 负责人: D R SANADI
• 金额: $ 5.45万
• 依托单位: BOSTON BIOMEDICAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-07-01 至 1986-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3268528
• 关键词: adenosinetriphosphatase; analytical chemistry; bacteria; chemical group; crosslink; electron microscopy; electron transport; enzyme complex; enzyme reconstitution; enzyme structure; heart metabolism; membrane reconstitution /synthesis; membrane structure; mitochondrial membrane; myocardium; oxidative phosphorylation; physical separation; radioimmunoassay; radiotracer; respiratory enzyme

In the mitochondrial oxidative phosphorylation system, the energy from the oxidative reactions is used for the generation of a H ion-gradient and associated membrane potential which subsequently drives the phosphorylation of ADP by Pi. Pi-ATP exchange, which is a part of the ADP phosphorylation reaction, is catalyzed by the vesicular energy transducing ATPase complex. Recent work from the laboratory has led to the identification of coupling factor B, a 15,000 dalton -SH containing protein, as a component of the Pi-ATP exchange activity catalyzed by the ATPase complex. The factor is not needed for the oligomycin sensitive ATPase activity. We propose to determine the location of the factor in the membrane - whether it is on the inner or outer surface of the inner mitochondrial membrane. Secondly, we will examine the effect of Factor B in depleted submitochondrial particles on the H ion-uptake associated with ATP breakdown and subsequent relaxation of the gradient due to leakage. A third aim will be to look for other functionally important groups in the protein using protein modifying agents.

在线粒体氧化磷酸化系统中，能量来自