GENETIC REGULATION OF PHOSPHOLIPID SYNTHESIS IN YEAST

磷脂合成的调节分析将在 单细胞真核生物酿酒酵母 研究 将涉及分离和表征突变体的缺陷， 合成和/或调节磷脂。 变种人会被雇佣 在监管研究，他们将被用来促进克隆 磷脂膜相关酶结构基因 生物合成 克隆的基因将用于研究 响应外源前体调节磷脂合成， 例如肌醇和胆碱。 克隆的基因也将用于 有助于研究这些蛋白质组装成其特定的 在细胞膜上的位置。 关于真核细胞控制细胞增殖的机制知之甚少。 膜脂的合成与进行中的膜协调 生物起源 然而磷脂，特别是含有肌醇的 磷脂与复杂的信号传导过程有关， 在控制细胞生长和增殖方面发挥作用， 真核生物 酵母细胞合成一种典型的真核混合物， 磷脂，使用的途径，这是类似于那些在较高的 真核生物 这种生物在基因上是容易控制的， 使用强大的分子遗传学。 因此，S.酿酒厂提出了一个 这是一个难得的机会，可以全面了解 膜磷脂合成的调控机制 简单真核生物的生物发生和细胞生长。

An analysis of regulation of phospholipid synthesis will be conducted in the unicellular eukaryotic organism, Saccharomyces cerevisiae. The studies will involve isolation and characterization of mutants with defects in the synthesis and/or regulation of phospholipids. The mutants will be employed in regulatory studies and they will be used to facilitate the cloning of structural genes encoding membrane associated enzymes of phospholipid biosynthesis. The cloned genes will be employed in studies of the regulation of phospholipid synthesis in response to exogenous precursors, such as inositol and choline. The cloned genes will also be used to facilitate studies of the assembly of these proteins into their specific locations in the cellular membranes. Little is known about the mechanisms by which eukaryotic cells control the synthesis of membrane lipids in coordination with ongoing membrane biogenesis. Yet phospholipids, particularly inositol containing phospholipids have been implicated in complex signalling processes which play a role in controlling cell growth and proliferation in higher eukaryotes. Yeast cells synthesize a typically eukaryotic mixture of phospholipids, using pathways which are similar to those in higher eukaryotes. The organism is genetically tractable, and can be manipulated using a powerful molecular genetics. Therefore, S. cerevisiae presents an unusual opportunity to develop a comprehensive understanding of the regulatory mechanisms controlling phospholipid synthesis during membrane biogenesis and cell growth in a simple eukaryote.