PHOSPHOLIPID METHYLTRANSFERASE IN S CEREVISAE & SCHIZOSACCHAROMYCES POMBE

酿酒酵母中的磷脂甲基转移酶

• 批准号: 6122471
• 负责人: SUSAN Armstrong HENRY
• 金额: --
• 依托单位: MELLON PITTS CORPORATION (MPC CORP)
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6122471
• 关键词: biological products; biomedical resource; genetics; lipids; nucleic acids; plants; proteins

Phospholipid(PL) methyltransferase catalyzes the last two sequential methylations in the synthesis of phosphatidylcholine(PC) in the phospholipid biosynthetic pathway in yeast, Saccharomyces cerevisiae. The activity of this pathway in other eukaryotic membranes has been implicated in diverse functions such as a regulator of lipid composition, modifier of membrane fluid properties, and as a transducer of receptor mediated signalling. One objective of the research described in this proposal is to purify and characterize PL methyltransferase from yeast. In addition, the regulation and the mechanism of localization of PL methyltransferase will be studied using biochemical and yeast molecular genetic techniques. The structural gene, OP13, encoding this enzyme in S. cerevisiae has been isolated in the Henry laboratory. Antibodies will be used to develop protocols for purifying the native PL methyltransferase and for analyzing its localization within various compartments of the yeast cell. In addition, the Schizosaccharomyces pombe homologue(CH01) to the S. cerevisiae OP13 gene has also been isolated in the Henry laboratory. An initial characterization of the S. pombe CH01 gene will be undertaken and used in a comparison study of PC biosynthesis and regulation in S. pombe and S. cerevisiae. Hopefully, the research proposed will lead to a better understanding of the function and regulation of the phospholipid methylation pathway in the synthesis of PC in yeast.

磷脂(PL)甲基转移酶催化后两种 磷脂酰胆碱(PC)合成中的顺序甲基化 酵母中磷脂的生物合成途径 酿酒。该途径在其他真核生物中的活性 膜与多种功能有关，如调节器 脂类成分，膜流体性质的改进剂，以及作为 受体介导的信号转导。目标之一就是 本方案描述的研究是对PL进行提纯和表征 酵母中的甲基转移酶。此外，该规定和 将对PL甲基转移酶的定位机制进行研究 使用生化和酵母分子遗传技术。这个 在酿酒酵母中编码这种酶的结构基因OP13已经被 被隔离在亨利实验室里。抗体将被用于开发 纯化天然磷脂酶甲基转移酶和 分析其在酵母不同隔室中的定位 手机。此外，裂殖酵母Pombe同源物(CH01)与 酿酒酵母op13基因也在亨利氏酵母中分离到。 实验室。庞氏链霉菌CH01基因的初步鉴定 将承担并用于PC生物合成的比较研究 以及对S.pombe和S.cerevisiae的调控。希望，这项研究 建议将导致更好地了解功能和 磷脂甲基化途径在磷脂合成中的调节 PC在酵母中。