PHOSPHOLIPID METHYLTRANSFERASE IN S CEREVISAE & SCHIZOSACCHAROMYCES POMBE

酿酒酵母中的磷脂甲基转移酶

• 批准号: 6282506
• 负责人: SUSAN Armstrong HENRY
• 金额: $ 1.19万
• 依托单位: MELLON PITTS CORPORATION (MPC CORP)
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6282506
• 关键词: biological products; biomedical resource; genetics; lipids; nucleic acids; plants; proteins

Phospholipid(PL) methyltransferase catalyzes the last two sequential methylations in the synthesis of phosphatidylcholine(PC) in the phospholipid biosynthetic pathway in yeast, Saccharomyces cerevisiae. The activity of this pathway in other eukaryotic membranes has been implicated in diverse functions such as a regulator of lipid composition, modifier of membrane fluid properties, and as a transducer of receptor mediated signalling. One objective of the research described in this proposal is to purify and characterize PL methyltransferase from yeast. In addition, the regulation and the mechanism of localization of PL methyltransferase will be studied using biochemical and yeast molecular genetic techniques. The structural gene, OP13, encoding this enzyme in S. cerevisiae has been isolated in the Henry laboratory. Antibodies will be used to develop protocols for purifying the native PL methyltransferase and for analyzing its localization within various compartments of the yeast cell. In addition, the Schizosaccharomyces pombe homologue(CH01) to the S. cerevisiae OP13 gene has also been isolated in the Henry laboratory. An initial characterization of the S. pombe CH01 gene will be undertaken and used in a comparison study of PC biosynthesis and regulation in S. pombe and S. cerevisiae. Hopefully, the research proposed will lead to a better understanding of the function and regulation of the phospholipid methylation pathway in the synthesis of PC in yeast.

磷脂（PL）甲基转移酶催化最后两个 磷脂酰胆碱（PC）合成中的顺序甲基化 酵母磷脂合成途径 啤酒。 该途径在其他真核生物中的活性 膜具有多种功能，如调节器 脂质组成，膜流体性质的改性剂，并作为 受体介导的信号转导。 的一个目的 本提案中描述的研究是纯化和表征PL 来自酵母的甲基转移酶 此外，条例和 PL甲基转移酶的定位机制将进行研究 使用生化和酵母分子遗传技术。 的 在S.酿酒厂已经 被隔离在亨利实验室里 抗体将用于开发 纯化天然PL甲基转移酶和 分析其在酵母的各个隔室中的定位 cell. 此外，粟酒裂殖酵母同源物（CH01）与 色葡萄在亨利酵母中也分离到了OP 13基因 实验室 对S.粟酒酵母CH 01基因 将进行并用于PC生物合成的比较研究 和调节作用。pombe和S.啤酒。 希望这项研究 建议将导致更好地了解功能， 磷脂甲基化途径的调节 酵母中的PC。