STRUCTURE AND REPLICATION OF DNA

DNA 的结构和复制

• 批准号: 3268679
• 负责人: ROSS B INMAN
• 金额: $ 20.4万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-01-01 至 1992-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3268679
• 关键词: Escherichia coli; bacterial genetics; bacteriophage lambda; computer simulation; electron microscopy; genetic manipulation; genetic mapping; membrane activity; nucleic acid denaturation; nucleic acid structure

Our major aim is to obtain new information about bacteriophage and bacterial DNA replication. Such studies are fundamental to the eventual understanding of DNA function in normal and abnormal growth, virus behavior and mutagenesis. Our studies utilize physical, biochemical and genetic techniques with major emphasis on physicochemical and electron microscopic methodology. We wish to determine why some replicators employ a bidirectional mode of DNA replication, other replicate unidirectionally and yet others switch their mode of replication. What evolutionary pressures favored these various strategies for DNA duplication? The segment of DNA that is responsible for the initiation of DNA replication will be studied in greater detail. We wish to find out exactly what properties this very important sequence has to have in order to be functionally active. Can other sequences take on such a function under unusual conditions? Minor aims are to continue studies on DNA helical stability. We wish to have more accurate understanding of the magnitude of effects produced by A+T or G+C rich segments on neighboring DNA sequences. Studies will also be continued on learning more about internal structure of bacteriophage. Our investigations will rely heavily on electron microscopic methods and to this end we wish to develop new techniques in this area and to extend the usefulness of electron microscopic partial denaturation mapping. One technique of immediate interest is development of a system capable of automatic DNA length measurement.

我们的主要目的是获得有关噬菌体的新信息， 细菌DNA复制 这些研究对于最终的 了解DNA在正常和异常生长中的功能，病毒行为 和诱变。 我们的研究利用物理，生物化学和遗传学 主要侧重于物理化学和电子显微镜技术 方法论 我们想知道为什么有些复制者 DNA复制的双向模式，其他复制单向和 还有一些改变了它们的复制模式。 什么样的进化压力 支持这些不同的DNA复制策略 的dna区段 负责启动DNA复制的基因将被研究 更详细地说。 我们希望弄清楚这到底是什么属性 重要的序列必须具备才能有功能活性。 可以 其他序列在不寻常的情况下也会有这样的功能？ 轻微 目的是继续研究DNA螺旋稳定性。 我们希望 更准确地理解A+T产生的影响的大小， 相邻DNA序列上的G+C丰富片段。 研究还将 继续学习更多关于噬菌体的内部结构。 我们 研究将严重依赖电子显微镜方法， 为此，我们希望在这一领域开发新技术，并扩大 电子显微镜部分变性作图的有用性。 一 当前感兴趣技术是开发一种系统，该系统能够 自动DNA长度测量。