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INTERACTION OF SPIN LABELS WITH TRANSITION METALS

INTERACTION OF SPIN LABELS WITH TRANSITION METALS
自旋标记与过渡金属的相互作用
批准号：
3270283
负责人：
Gareth R Eaton
金额：
$14.1万
依托单位：
UNIVERSITY OF DENVER (COLORADO SEMINARY)
依托单位国家：
美国
项目类别：
财政年份：
1977
资助国家：
美国
项目状态：
已结题
起止时间：
1977-07-01 至 1989-11-30
来源：
https://reporter.nih.gov/project-details/3270283
关键词：
adenosinetriphosphatase chemical models chemical synthesis computer simulation electron spin resonance spectroscopy heavy metals ligands metal complex nitrous oxide urease

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英文摘要
The goal of this research is to obtain metrical information from continuous wave and pulsed EPR spectroscopy of interacting spin systems in biological molecules. EPR transition energies, transition probabilities, and relaxation times will be calculated for spin systems of interest. These calculations will be calibrated with discrete metal complexes containing paramagnetic metals and nitroxyl spin labels. Exchange and dipolar contributions to the spin-spin interaction will be measured. The limits of applicability of various approaches to measuring spin-spin distances will be determined. These results will be applied to the nickel metalloenzyme urease, the copper-containing plastocyanin, and to CaATPase.
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