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Spinning disk microscope for Wolfson Light Microscopy Facility

用于 Wolfson 光学显微镜设备的转盘显微镜

基本信息

批准号：
BB/V019368/1
负责人：
Elizabeth Smythe
金额：
$ 34.4万
依托单位：
University of Sheffield
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2021
资助国家：
英国
起止时间：
2021 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FV019368%2F1
关键词：
Spinning disk microscope Wolfson Light

项目摘要

How components interact within cells is key to how cells behave and this in turn affects the organisation of tissues and ultimately the whole organism. Seeing how these interactions occur is key to our understanding of how a healthy organism forms and continues to stay healthy, and what happens when things go wrong in disease. Often visualisation of biological events causes us to formulate new hypotheses. Researchers thus use microscopy extensively to understand how cells, tissues and organisms function. Frequently, fluorescent labels are attached to components of interest which allows them to be observed in living cells. The time-scale of biological processes is highly variable: some processes happen very quickly, meaning that images need to be acquired speedily while others occur over a much longer time-scale of minutes to hours. There are a number of challenges to capturing fluorescence images because the exposure of cells to light can cause phototoxicity which damages cells and causes aberrant behaviour. Additionally, some fluorescent labels can be quite dim which makes the fluorescent signal difficult to detect.The Wolfson Light Microscopy Facility (LMF) at the University of Sheffield houses a range of microscopes that are optimised for particular applications. Spinning disk confocal microscopy allows imaging of fast dynamic events in 3 dimensions. Our current spinning disk confocal microscope is extensively used to understand how normal physiology is regulated, and what changes as organisms age. It is also used in experiments with model organisms such as zebrafish and fruit flies which help us to understand how the immune system works. Our existing system is however now greater than 12 years old and is vulnerable because the original parts are no longer supported by the manufacturers. The aim of this proposal is to install a replacement spinning disk microscope. This will not only accommodate our existing needs but will open up a range of exciting new possibilities because the new generation spinning disk confocal microscopes offer significantly enhanced performance in terms of speed, sensitivity, and low phototoxicity. These advantages, coupled with the ability to capture wide fields-of-view, mean that this is a very powerful instrument which will have a wide range of uses within the LMF. We have identified projects ranging from cell and developmental biology, immunology and microbiology which will benefit from the acquisition of a state-of-the-art spinning disk confocal microscope.

细胞内成分如何相互作用是细胞行为的关键，这反过来又影响组织的组织，并最终影响整个生物体。了解这些相互作用是如何发生的，对于我们理解健康有机体如何形成并持续保持健康，以及当疾病出现问题时会发生什么至关重要。生物事件的可视化常常促使我们提出新的假设。因此，研究人员广泛使用显微镜来了解细胞、组织和生物体的功能。通常，荧光标记附着在感兴趣的成分上，从而可以在活细胞中观察它们。生物过程的时间尺度变化很大：一些过程发生得非常快，这意味着需要快速获取图像，而另一些过程则发生在几分钟到几小时的更长的时间范围内。捕获荧光图像存在许多挑战，因为细胞暴露在光下会引起光毒性，从而损害细胞并导致异常行为。此外，一些荧光标记可能非常暗淡，这使得荧光信号难以检测。谢菲尔德大学的 Wolfson 光学显微镜设施 (LMF) 拥有一系列针对特定应用进行优化的显微镜。转盘共焦显微镜可以对 3 维快速动态事件进行成像。我们当前的转盘共焦显微镜广泛用于了解正常生理机能如何调节，以及生物体衰老时会发生什么变化。它还用于斑马鱼和果蝇等模型生物的实验，帮助我们了解免疫系统的工作原理。然而，我们现有的系统已经使用了 12 年以上，并且很容易受到攻击，因为制造商不再支持原始部件。该提案的目的是安装一个替换的转盘显微镜。这不仅将满足我们现有的需求，还将开辟一系列令人兴奋的新可能性，因为新一代转盘共焦显微镜在速度、灵敏度和低光毒性方面显着增强了性能。这些优点，再加上捕获宽视场的能力，意味着这是一种非常强大的仪器，将在 LMF 中具有广泛的用途。我们已经确定了细胞和发育生物学、免疫学和微生物学等项目，这些项目将受益于购买最先进的转盘共聚焦显微镜。

项目成果

期刊论文数量（7）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Macrophage subpopulation identity in Drosophila is modulated by apoptotic cell clearance and related signalling pathways.

DOI：
10.3389/fimmu.2023.1310117
发表时间：
2023
期刊：
Frontiers in immunology
影响因子：
7.3
作者：
通讯作者：

Aberrant Wnt activation in recurrent genetically variant human pluripotent stem cells impairs cardiomyocyte differentiation and phenotype

复发性遗传变异人多能干细胞中异常的 Wnt 激活损害心肌细胞分化和表型

DOI：
10.1101/2023.12.12.571269
发表时间：
2023
期刊：
影响因子：
0
作者：
Wing T
通讯作者：
Wing T

A neuroepithelial wave of BMP signalling drives anteroposterior specification of the tuberal hypothalamus.

DOI：
10.7554/elife.83133
发表时间：
2023-02-10
期刊：
eLife
影响因子：
7.7
作者：
Chinnaiya K;Burbridge S;Jones A;Kim DW;Place E;Manning E;Groves I;Sun C;Towers M;Blackshaw S;Placzek M
通讯作者：
Placzek M

Macrophage transplantation rescues RNASET2-deficient leukodystrophy by replacing deficient microglia in a zebrafish model

巨噬细胞移植通过替换斑马鱼模型中缺陷的小胶质细胞来挽救 RNASET2 缺陷的脑白质营养不良