SPRINT: A SuPer-Resolution time-resolved ImagiNg and specTroscopy facility for rapid biomolecular analysis

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It is essential to understand the structure and dynamics of biomolecules if we are to exploit them for medical or industrial applications. Molecules such as proteins and enzymes are dynamic, and understanding their interactions within cell pathways and biological processes can provide new insight into fundamental mechanisms and support the development of new therapeutic diagnostics and drugs. We usually require the use of a range of complex equipment to understand and characterise biomolecules fully. One common way to visualise molecular interactions is through the use of specialised microscopes; however, this approach can only resolve structures that are greater than 250 nm apart (0.00025 mm). Proteins and other molecules found in cells are much smaller than this (around 0.00001 mm), and therefore to resolve single proteins and their interactions requires access to more advanced microscopy techniques, commonly referred to as "super-resolution" microscopy. This application proposes to establish a Scottish microscopy facility that adopts super-resolution techniques, in particular a 'quantum-ruler' (called FLIM-FRET, capable of measuring molecular interactions happening within 0.00001 mm) and a method called FCS that allows single molecules and their behaviours to be studied in microscopic regions of living cells. Access to this new facility will have a significant impact on academic researchers across Scotland. This facility will also facilitate the training of young early-stage researchers in cutting-edge technology. It will also support the training of doctoral students ensuring the next generation of scientists have the skills required to deliver significant new scientific breakthroughs to help build the UK capability and sustainability in the area of biomolecular interactions.